担载b-FGF的PLGA微球复合明胶支架的体外释放研究

In Vitro Release Research of Gelatin Scaffold with PLGA Microspheres Containing Basic Fibroblast Growth Factor

目的：研究担载碱性成纤维细胞生长因子（b-FGF）微球复合明胶支架的外形特征、孔径、孔隙率及体外释放动力学,以期构建具有缓释功能、高孔隙率的担载细胞因子的新型复合明胶支架。方法：本文利用冷冻相分离法和S/O/W法先将b-FGF水溶液包裹于PLGA微球中,然后埋置于明胶溶液中制备为多孔复合明胶支架。分别对微球的形态和复合明胶支架的基本形态、孔径、孔隙率进行表征,通过Elisa法测定b-FGF在复合明胶支架中的体外释放行为。结果：制备成形态良好的三维复合明胶支架,其孔隙率为82.90%±1.45%,孔径范围为150~300μm,复合明胶支架中b-FGF在体外缓慢释放20余天。结论：担载蛋白微球复合明胶支架不仅满足组织工程支架的要求,还能有效缓释细胞因子,为细胞和组织生长提供良好的微环境,为进一步应用于组织工程领域提供了可能。

Objective： To investigate the characterization, the pore size and the porosity ratio of gelatin scaffold with PLGA microspheres containing basic fibroblast growth factor and its release behavior in vitro of b-FGF, for constructing a new composite gelatin scaffold loaded growth factor with sustain-released and high porosity ratio. Methods： By the frozen phase separation method and S/O/W method b-FGF aqueous solution prepared into PLGA microspheres, then embedded into gelatin-scaffold to form the final composite scaffold. The morphology of microspheres and gelatin scaffold, the pore size and the porosity ratio of gelatin scaffold were characterized and release kinetic of b-FGF in composite gelatin scaffold was detected by Elisa method in vitro. Results： The composite three-dimensional（3D） scaffold had good morphology with the porosity ratio of 82.90%±1.45% and the pore sizes ranging from 150 to 300 μm. In vitro,b-FGF in composite gelatin scaffold had sustained release over 20 days. Conclusion： The gelatin scaffold with PLGA microspheres containing growth factor is not only satisfied the requirements of tissue engineering scaffolds, but also sustained release growth factor for providing a microenvironment to cells and tissue growth and further application to tissue engineering in future.