摘要
目的了解临床铜绿假单胞菌I类整合子消毒剂—磺胺耐药基因(qac E△-sul1)携带情况及阳性菌株对聚维酮碘的抗性,为预防医院感染提供依据。方法检测临床标本分离到的铜绿假单胞菌(均为多重耐药)qac E△-sul1基因;分别测试基因阳性菌株与基因阴性菌株对聚维酮碘的最低抑菌浓度(MIC)/最低杀菌浓度(MBC);采用不同浓度的聚维酮碘作用不同时间于基因阳性菌株与基因阴性菌株,分别计算杀灭率并比较。结果400株多重耐药铜绿假单胞菌qac E△-sul1基因阳性者39株,占9.75%。0.1 m L菌悬液的qac E△-sul1基因阳性和阴性菌株MIC和MBC一致为250 mg/L;2 000 mg/L的聚维酮碘作用于基因阳性菌株与阴性菌株各15 s,杀灭率分别为99.84%和99.90%。结论 qac E△-sul1基因存在于临床分离的多重耐药铜绿假单胞菌中,其阳性菌株与阴性菌株对聚维酮碘的抗性无明显差异;2 000 mg/L聚维酮碘作用于多重耐药铜绿假单胞菌qac E△-sul1阳性与阴性菌株15 s均不能100%将其杀灭,提示临床应严格正确掌握聚维酮碘的作用消毒时间。
Objective To explore the carrying status of class I integron disinfectant- sulfanilamide resistance gene( qac E△- sul1) in the strains of pseudomonas aeruginosa and its resistance to povidone iodine. Methods The gene( qac E △- sul1) was detected in clinical strains of pseudomonas aeruginosa. The minimal inhibitory concentrations( MIC) and the minimum bactericidal concentrations( MBC) of povidone iodine were detected and the changes of the bactericidal rates of povidone iodine with different concentrations and times were observed in qac E △- sul1 positive strains. Results A total of 39 positive strains were detected from the clinical strains of pseudomonas aeruginosa( 9. 8%). MIC and MBC of povidone iodine in 0. 5 ml qac E △- sul1 positive strains was both 500 mg / L. The bactericidal rate of povidone iodine with 2 000 mg / L in 15 s was 99. 84 % and 99. 90 % in qac E △- sul1 positive and negative strains,respectively.Conclusions The resistance gene( qac E △- sul1) found in pseudomonas aeruginosa had no effects on the resistance to povidone iodine. Povidone iodine with 2000 mg / L in 15 s was failed to kill pseudomonas aeruginosa completely and more attention should be paid on effective time of povidone iodine.
出处
《浙江预防医学》
2015年第11期1106-1109,共4页
Zhejiang Journal of Preventive Medicine
基金
浙江省卫生厅资助项目(2013KYA196)
