四逆散及其拆方对实验性溃疡性结肠炎大鼠SOCS1·STAT6活性的影响

Sini powders and different compatibilities influence on SOCS1·STAT6 in experimental ulcerative colitis rats

目的从IL-4/STAT6信号通路入手,以该通路负反馈调节因子SOCS1和关键因子STAT6为检测指标,多角度探查溃疡性结肠炎的发生与IL-4的关系。方法将实验动物分为6组,采用免疫法造模,用免疫组化法检测大鼠SOCS1、STAT6的活性。结果模型组实验大鼠SOCS1活性(0.485 5±0.178 0)显著低于正常组(0.655 2±0.187 4),P<0.05;而STAT6活性(0.803 0±0.208 2)则明显高于正常组(0.589 3±0.191 9),P<0.05。四逆散能显著增加实验大鼠SOCS1活性(0.576 5±0.208 0)(P<0.05),降低STAT6活性(0.579 8±0.152 6)(P<0.05);柴芍配伍(0.562 5±0.275 7)对SOCS1活性的影响与四逆散(0.576 5±0.208 0)比较差异无统计学意义,对STAT6活性(0.529 4±0.144 3)表达的影响显著优于四逆散(0.579 8±0.152 6)(P<0.05);芍枳甘配伍(0.616 9±0.200 6)对STAT6活性的影响作用明显,与四逆散(0.579 8±0.152 6)比较差异无统计学意义;柴枳甘配伍对SOCS1(0.509 1±0.227 9)、STAT6(0.756 1±0.263 6)的影响与模型组(0.485 5±0.178 0;0.803 0±0.208 2)比较差异无统计学意义。结论四逆散能够通过刺激SOCS1活性,抑制STAT6活性表达来调节IL-4/STAT6通路,进而干预实验性溃疡性结肠炎。其中,柴芍配伍作用明显;芍枳甘配伍对STAT6活性的影响作用明显,但对SOCS1的影响不显著;柴枳甘配伍对SOCS1、STAT6的影响均不显著。

Objective To explore the relation with IL-4 and UC through multi angles,starting with the IL-4 / STAT6 pathway. Methods The experimental animals were divided into 6 groups. We made the model in immune method,de-tected activity of SOCS1 and STAT6 in rats with immune. Results The activity SOCS1 in model group of rats（0. 485 5 ± 0. 178 0）were significantly lower than that of normal group（0. 655 2 ± 0. 187 4）（P 〈 0. 05）,but the activity of STAT6（0. 803 0 ± 0. 208 2）were significantly higher than that in the normal group（0. 589 3 ± 0. 191 9） （P 〈 0. 05）. Sinisan Powders can significantly increase the activity of SOCS1（0. 576 5 ±0. 208 0）in experimental rats （P 〈0. 05）,decrease STAT6 activity（0.579 8 ±0.152 6）（P 〈0. 05）. The effects of Chaishao compatibility（0. 562 5 ± 0.275 7）was no significant difference with SOCS1 and Sinisan（0. 576 5 ±0. 208 0）Powders（P 〉0. 05）,the activity of STAT6（0.529 4 ±0.144 3）was significantly better than the Sinisan（0.579 8 ±0.152 6）Powders（P 〈0.05）. Shaozhigan compatibility effects on activity of STAT6（0. 616 9 ± 0. 200 6）significantly. Chaizhigan compatibility had no significant difference with SOCS1（0.509 1 ±0.227 9）,STAT6（0.756 1 ±0.263 6）and the model group（0.485 5 ±0.178 0;0.803 0 ±0.208 2）in effects（P 〉0.05）. Conclusion Sinisan Powders can regulate expression of the IL-4/ STAT6 pathway by stimulating the activity of SOCS1,inhibiting the activity of STAT6,then intervention experiment UC. And the effect of Chaishao compatibility was the best obvious;the effects of Shaozhigan compatibility was obvious on activity of STAT6,but not on SOCS1. The effect of Chaizhigan compatibility were not obvious on SOCS1 and STAT6.