不明原因复发性流产病人流产组织绒毛及血清中ICAM-1、VCAM-1和TNF-α表达

THE EXPRESSIONS OF ICAM-1,VCAM-1 AND TNF-αIN VILLUS AND SERUM OF PATIENTS WITH UNKNOWN-ORIGIN RECURRENT SPONTANEOUS ABORTION

目的探讨细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1（VCAM-1）和肿瘤坏死因子-α（TNF-α）在不明原因复发性流产病人血清及流产组织绒毛中的表达情况。方法选取30例不明原因复发性流产病人（实验组）及30例正常早期妊娠人工流产孕妇（对照组）,采用酶联免疫吸附试验测定两组病人血清中ICAM-1、VCAM-1和TNF-α的水平,并采用免疫组化方法检测两组病人流产组织绒毛中ICAM-1、VCAM-1和TNF-α的表达。结果实验组血清中ICAM-1、VCAM-1及TNF-α水平与对照组比较,差异有显著性（t=-3.916～4.261,P〈0.01）;实验组流产组织绒毛中ICAM-1、VCAM-1及TNF-α表达与对照组比较,差异有显著性（Zc=82.0～762.5,P〈0.001）。结论血清及流产组织绒毛中ICAM-1、VCAM-1和TNF-α表达异常可能与复发性流产的发生密切相关。

Objective To study the expressions of ICAM-1,VCAM-1and TNF-αin serum and villus of abortion tissue in patients with unknown-origin recurrent spontaneous abortion（RSA）. Methods Thirty patients with unknown-origin RSA（experimental group）and 30 normal early pregnancy underwent artificial abortion（control group）were enrolled.Serum levels of ICAM-1,VCAM-1and TNF-αwere determined in both groups using enzyme-linked immunosorbent assay,and the expressions of ICAM-1,VCAM-1and TNF-αin villus of abortion tissue detected by immunohistochemical method. Results A comparison between experimental group and control group showed that the differences were significant with regard to serum ICAM-1,VCAM-1and TNF-α（t=-3.916-4.261,P〈0.01）and that in villus of abortion tissue（Zc=82.0-762.5,P〈0.001）. ConclusionThe abnormal expressions of ICAM-1,VCAM-1and TNF-αin serum and villus of miscarriage tissue in patients with unknown-origin recurrent miscarriage hint that the above factors may have a close association with the condition.