胃癌组织中VEGF-C、CD105和RhoC mRNA的表达及意义

The signification of VEGF-C,CD105 and RhoC mRNA expression in gastric carcinoma

目的探讨血管内皮生长因子C(VEGF-C)、CD105和Ras同源蛋白C(RhoC)mRNA在胃癌组织中的表达及其与淋巴结转移的关系。方法采用实时荧光定量逆转录-聚合酶链反应(Real-time PCR)检测62例胃癌组织及62例癌旁周围组织中VEGF-C、CD105和RhoC的表达。结果 VEGF-C mRNA在胃癌组织中的表达量(0.367±0.073)显著高于癌旁正常胃组织(0.121±0.058),P<0.01。胃癌组织中VEGF-C蛋白阳性表达率为53.2%(33/62)显著高于癌旁正常胃组织中阳性表达率8.1%(5/62),P<0.01。胃癌组织中VEGF-C mRNA及蛋白的表达与有无淋巴结转移、TNM不同分期差异有统计学意义(P<0.01),而与性别、年龄、肿瘤直径、分化程度、浸润深度差异无统计学意义(P>0.05)。CD105 mRNA在胃癌组织中的表达量(0.419±0.072)显著高于癌旁正常胃组织(0.214±0.058),P<0.01。胃癌组织中CD105蛋白的阳性表达率为88.1%(52/62)显著高于癌旁正常胃组织中的阳性表达率11.3%(7/62),P<0.01。胃癌组织中CD105 mRNA及蛋白的表达与浸润深度、有无淋巴结转移、TNM不同分期差异有统计学意义(P<0.01),而与性别、年龄、肿瘤直径、分化程度差异无统计学意义(P>0.05)。RhoC mRNA在胃癌组织中的表达量(1.122±0.211)显著高于癌旁正常组织(0.868±0.199),P<0.01。胃癌组织中Rho C蛋白阳性表达率为75.8%(47/62)显著高于癌旁正常胃组织中阳性表达率22.6%(14/62),P<0.01。胃癌组织中RhoC mRNA及蛋白的表达与浸润深度、有无淋巴结转移、TNM不同分期差异有统计学意义(P<0.01),而与性别、年龄、肿瘤直径、分化程度差异无统计学意义(P>0.05)。结论 VEGF-C、CD105和Rho C可能在胃癌的发生、发展和淋巴结转移中发挥重要作用。

Objective To explore the expression of vascular endothelial growth factor-C （VEGF-C）, CD105 and RhoC in gastric cancer. Methods The real-time reverse transcription polymerase chain reaction （RT-PCR） was used to determine the expression of VEGF-C ,CD105 and RhoC mRNA in 62 tissue samples of gastric cancer and 62 specimens in adjacent carcinoma tissue. Results The expression of VEGF-C mRNA in gastric cancer tissues was obviously higher than that in normal control group（0. 367 ±0.073 vs. 0. 121 ± 0.058, P 〈 0.01 ） ;the positive expression ratios of VEGF-C in gastric cancer tissues and in control group were 53.2% （33/62） and 8.1% （5/62）, and significant differences were found between the two groups（ P 〈0.01 ）. The expression of VEGF-C mRNA and protein in gastric cancer had significant correlation with lymph node metastasis and TMN staging of tumor（P 〈0.01 ） ,while no significant difference were found in gender, age, the size of tumor , histological grade and the depth of invasion （P 〉 0.05）. The expressions of CD105 mRNA in gastric cancer tissues were obviously higher than that in normal control group （0. 419 ±0. 072 vs. 0. 214 ± 0. 058, P 〈 0.01 ） ;the positive expression ratios of CD105 in gastric cancer tissues and in control group were 88.1% （52/62） and 11.3% （7/62）, and significant differences were found between the two groups（ P 〈 0.01 ）. The expression of CD105 mRNA and protein in gastric cancer had significant correlation with the depth of invasion,lymph node metastasis and TMN staging of tumor（ P 〈 0. 01 ） , while no significant difference were found in gender, age, the size of tumor and histological grade（ P 〉 0. 05 ）. The expressions of RhoC mRNA in gastric cancer tissues were obviously higher than that in normal control group（ 1. 122 ±0. 211 vs. 0. 868 ± 0. 199,P 〈 0.01 ） ;the positive expression ratios of RhoC in gastric cancer tissues and in control group were 75.8 % （47/62） and 22.6% （ 14/62 ）, and significant differences were found between the two groups （ P 〈 0. 01 ）. The expression of RhoC mRNA and protein in gastric cancer had significant correlation with the depth of invasion,lymph node metastasis, and TMN staging of tumor（ P 〈 0.01 ） , while no significant difference were found in gender, age, the size of tumor, the depth of invasion and histological grade （ P 〉0. 05 ）. Conclusion VEGF-C, CD 105 and RhoC may play an important role in the pathogenesis, development and lymph node metastasis of gastric carcinoma.