细微玉米粉同步糖化动力学分析

Study on the kinetics of simultaneous liquefaction and saccharification with micronized corn flours

采用同步液化糖化法对细微玉米粉进行糖化处理,确定细微玉米粉同步糖化速率模型,考察p H和温度对同步糖化反应速率常数和葡萄糖收率的影响。实验结果表明,细微玉米粉的同步糖化反应为2级反应;p H对反应速率常数和葡萄糖收率具有显著性影响（p〈0.05）,温度对葡萄糖收率影响不大（p〉0.05）,对反应速率常数影响显著（p〈0.05）,在60℃、p H6.0条件下,葡萄糖收率最高,达到84.6%,反应速率常数为0.0088（g/L）-1·h-1;在30~70℃的温度范围内,糖化速率常数与温度的关系可用Arrhenius方程式表示,其反应活化能为3.3×104J/mol。玉米粉同步糖化动力学模型的建立及其活化能分析将为其在生产上的应用提供很好的指导意义。

In order to establish the rate model of simultaneous liquefaction and saccharification（ SILS）,simultaneous enzymatic hydrolysis of the micronized corn flour samples were carried out using commercially available a- amylase and glucoamylase,and effect of p H and temperature on rate constant and glucose yield were studied.Experimental results showed that the kinetics of SILS accorded with the character of the two order reaction. Both the glucose yield and reaction rate constant were affected significantly by the p H of feed mixture（ p 〈0.05）. The effect of SILS temperature on the glucose yield was not significant（ p 〉0.05）,but it had significant effect on the reaction rate constant（ p〈 0.05）. Under the condition of temperature at 60 ℃ and p H at 6.0,the highest glucose yield reached 84.6% and the reaction rate constant was 0.0088（ g / L）- 1·h- 1.In the temperature range of 30~70 ℃,the relationship between reaction rate constant and temperature could be expressed by Arrhenius equation. The activation energy was 3.3 × 104J·mol- 1for the SILS of micronized corn flours. The study on the Kinetics and activation energy of SILS could provide a good guiding significance for application in production.