18株植物内生真菌的分离纯化及鉴定

Isolation and identification of 18 strains of endophytic fungi from plants

植物内生真菌生活环境特殊、种类多,通过大量的分离和筛选得到有价值的内生真菌,可以为新型天然产物的获得提供更丰富的来源。本实验随机采摘了12种植物,采用组织分离法从中有选择性地分离纯化得到了18株内生真菌,通过28S r DNA序列测定和系统发育树分析,对18株真菌进行了鉴定。结果表明,这18株内生真菌主要属于Cladosporium,Paramicrothyrium,Mycosphaerella,Colletotrichum,Corynespora,Sarocladium,Guignardia,Phoma,Pseudocercospora,Collembolispora,Alternaria,Glomerella等属,说明了植物内生真菌的丰富多样性。得到的18株内生真菌中有3株不常见真菌和一株新真菌,其次级代谢产物值得进一步研究。

Plant endophytic fungi have the characteristics of special living environment and variety. By a large number of isolation and screening to obtain valuable endophytic fungi,it can provide more sources for novel natural products.In this study,12 kinds of plants were randomly picked.18 strains of endophytic fungi were isolated by traditional artificial separation method.These endophytic fungi were identified using 28 S r DNA sequencing and phylogenies. The results showed that 18 strains of endophytic fungi mainly belonged to Cladosporium,Paramicrothyrium, Mycosphaerella, Colletotrichum, Corynespora, Sarocladium, Guignardia, Phoma,Pseudocercospora,Collembolispora,Alternaria and Glomerella sp.,indicating the rich diversity of endophytic fungi.Among these fungi,there were three rare strains and a new strain of fungi.The secondary metabolites of these fungi were worth further studying.