响应面优化酶促油酸甘油酯化制备甘三酯工艺的研究

Optimization of enzymatic esterification of oleic acid with glycerol for the preparation of triglycerides by response surface methodology

采用响应面法对脂肪酶Novozym 435在无溶剂体系中催化甘油和油酸酯化反应合成甘三酯的反应条件进行了研究。结果表明:底物摩尔比、反应时间、反应温度和加酶量都对甘三酯的含量有影响(p<0.05),其中底物摩尔比影响最为显著(p=0.0104)。经过响应面优化得到的最佳反应条件为:底物摩尔比(油酸∶甘油)2.92∶1,反应时间12 h,反应温度98℃,加酶量(以占油酸和甘油总质量的百分比计)2.96%。在最优条件下,甘三酯含量可达到90.88%±1.56%,酯化度达到93.09%±1.42%。

The triglycerides were synthesized from glycerol and oleic by Novozym 435 lipase catalysis in a solventfree system,and the reaction conditions were optimized by response surface methodology（ RSM）. The substrate molar ratio,reaction time,reaction temperature and enzyme load strongly affected triglyceride content（ p 〈0.05）,and substrate molar ratio had the most significant effect on triglyceride content（ p = 0.0104）. The optimum conditions were as follows： substrate molar ratio（ oleic acid / glycerol） 2.92 ∶ 1,reaction time 12 h,reaction temperature 98 ℃,and enzyme load 2.96%（ based on the total weight of oleic acid and glycerol）. Under the optimum conditions,triglyceride content was 90.88% ± 1.56%,esterification degree was 93.09% ± 1.42%.