冠瘤海鞘抗菌活性筛选及低极性组分气相色谱-质谱分析

Antibacterial activities and GC- MS analysis of low polar components from Styela canopus Savigny

目的:研究冠瘤海鞘的抗菌活性部位及有效成分。方法:采用抑菌圈法和最小杀菌浓度法,对中国南海冠瘤海鞘的乙醇提取物和不同极性部位进行了抗菌活性测定,并采用GC-MS技术对其石油醚相低极性组分进行了化学成分分析。结果:结果显示,抗菌活性成分主要在冠瘤海鞘的正丁醇相,其次是石油醚相;从冠瘤海鞘石油醚相中鉴定出14个化合物,主要为脂肪类和甾醇类化合物;从甾体组分中鉴定出7个化合物,主要为胆甾醇,其中4个为首次从冠瘤海鞘中分离鉴定。结论:冠瘤海鞘的正丁醇相和石油醚相具有明显的抗菌活性,石油醚相以胆甾醇类化合物为主。

Objective： To screen the antibacterial fractions and constituents of Styela canopus Savigny. Method： The antibacterial activities of the ethanolic extract and organic fractions of S. canopus from the South China Sea was evaluated by diameter of antibacterial circle and the minimum bactericidal concentration（ MBC）,and the low- polar compounds in the petroleum ether fraction were also analyzed by gas chromatography- mass spectrometry（ GC-MS）. Results： The results showed that the antibacterial substances were mainly in the n- butanol and petroleum ether fraction,and 14 compounds were identified by GC- MS from petroleum ether fraction,most of which were lipids and sterols. Seven compounds were identified by GC- MS from the sterol component,and cholesterol was the dominant sterol. Four compounds were identified for the first time from S. subcrenata.Conclusion： The antibacterial activities of n- butanol and petroleum ether fraction from S. canopus were obvious,and cholesterol was the dominant sterol of petroleum ether fraction.