制备抗五氯酚钠单克隆抗体及其应用于酶联免疫试剂盒的初步研究

Study on the production of pentachlorophenoi- na monoclonal antibodies and its enzyme linked immunosorbent assay kit for rapid detection

本文通过五氯酚钠与4-溴丁酸乙酯经过一系列反应,得到半抗原,制备出抗五氯酚钠单克隆抗体,然后利用酶联免疫技术研制了一种快速检测鱼肉、虾肉中的五氯酚钠试剂盒,经过测试,该试剂盒对鱼肉、虾肉样本的检测限为0.75μg/kg,IC50为0.31μg/L,平均回收率为71.5%~104.7%,试剂盒的标准曲线范围为0.05~4.05μg/L,批内、批间相对标准偏差小于10%,稳定性良好。

The synthesized pentachloropheno I- na hapten was prepared from a sequence of reactions that used pentachloropheno I- Na and 4- Bromobutyric acid ethyl ester. And pentachloropheno I- Na monoclonal antibodies were prepared by pentachloropheno I- Na antigen.Then the limit of detection and relative standard deviation（ RSD）of pentachloropheno I-Na enzyme linked immunosorbent assay kit was studied.The limit of detection was 0.75 μg / kg in fish and shrimp,with the IC50 values of 0.31 μg / L.The average recoveries were between 71.5% and 104.7%.The standard curve ranged from 0.05 to 4.05 μg / L,and RSD was less than 10%.The stability tests results revealed that the kit can be kept for a long time.