结肠癌和肝癌与多环芳烃类致癌物的相关性

CORRELATION BETWEEN COLON AND LIVER CANCER AND POLYCYCLIC AROMATIC HYDROCARBONS

目的了解多环芳烃（PAHs）与人类结肠癌和肝癌的相关性。方法应用超声固相萃取和高效液相色谱分析方法,检测30例结肠癌和15例肝癌组织及其癌旁组织PAHs含量,以35例非癌结肠组织和30例非癌肝组织作为对照。结果共检出5种PAHs,分别是菲、芘、2-甲基蒽和苯并（a）芘、2-甲基萘。结肠癌组织5种PAHs含量与其癌旁组织比较差异无统计学意义（P〉0.05）;结肠癌及其癌旁组织中芘、2-甲基蒽和苯并（a）芘的含量高于非癌结肠组织,差异有统计学意义（F=3.16～5.08,P〈0.05）;2-甲基萘、菲含量与非癌结肠组织比较差异无统计学意义（P〉0.05）。肝癌及其癌旁组织、非肝癌组织标本中5种PAHs含量差异无统计学意义（P〉0.05）。结论人类结肠组织和肝组织中均存在PAHs,PAHs与人类结肠癌的发生发展可能有一定相关性。

Objective To study the correlation between polycyclic aromatic hydrocarbons（PAHs）and colon and liver cancer. Methods Employing ultrasound solid-phase extraction and high performance liquid chromatography（HPLC）,PAHs in30 cases of colon cancer and 15 cases of liver cancer and their para-cancer tissue were detected,and tissue taken from 30 cases of non-colon cancer and 30non-liver cancer served as controls. Results A total of five kinds of polycyclic aromatic hydrocarbons-2-methyl naphthalene,phenanthrene,pyrene,2-methyl anthracene and benzo（a）pyrene-were detected.In colon cancer tissue,the contents of the five kinds of PAHs were not significantly different from that in its para-cancer tissue（P〉0.05）,and the contents of pyrene,2-methyl anthracene and benzo（a）pyrene in colon cancer tissue and its para-cancer tissue were higher than that of non-colon cancer tissue,the differences being statistically significant（F=3.16-5.08,P〈0.05）,and the differences of 2-methyl naphthalene and phenanthrene between colon cancer tissue and non-colon cancer were not significant（P〉0.05）.In the three kinds of liver specimens,the differences of the contents of the five kinds of PAHs had no statistical significance（P〉0.05）.Conclusion Polycyclic aromatic hydrocarbons are found in tissue of human colon and liver,which are probably associated with the occurrence and development of colon cancer in human being.