江苏省输入性登革热1例病毒分离鉴定及分子特征分析

Identification and molecular characteristics analysis of dengue virus isolated from an imported dengue fever case in Jiangsu province

目的 :从江苏省2012年4月1例菲律宾输入的疑似登革热患者急性期血清中分离病毒并分析其分子生物学特征,以找出病原学证据。方法:收集患者血清样本,采用胶体金法检测登革病毒的Ig M、Ig G抗体;用C6/36细胞分离病毒,对细胞病变的标本进行RT-PCR及荧光定量PCR检测登革病毒RNA和鉴定型别;采用高通量测序技术对该分离株进行编码区基因测序,用MEGA6.0软件对其进行序列比对和进化分析。结果:从患者血清样本中检测到登革病毒的Ig M抗体,分离到的毒株经鉴定为登革病毒1型,与美国Haw O3663毒株的核苷酸同源性>98%。结论:该病例是由登革病毒1型引起的输入性病例。

Objective：To identify the virus strain isolated from a suspected dengue case imported to Jiangsu province from Philippines in April 2012 and analyze its molecular characteristics. Methods：The serum sample collected from the patient was detected for Ig M and Ig G by colloidal gold method. The sample was further inoculated in C6 / 36 cells for virus isolation. Dengue nucleic acid was detected in the isolates via a stable cytopathic effect on C6 / 36 cells according to reverse translation-PCR and realtime PCR. Genome sequence information from the isolated strain was obtained by next-generation high-throughput sequencing.Nucleotide alignment and phylogenetic tree construction were conducted by MEGA6.0 software. Results：Ig M and RNA of type 1dengue virus were detected in the serum sample. The nucleotide homology with dengue virus 1 isolated from American Haw O3663 was more than 98%. Conclusion：The imported case of fever was caused by the dengue virus type 1.