利用高通量测序对三江平原小叶章湿地土壤细菌多样性的研究

Study on Bacterial Diversity of Deyeuxia angustifolia Wetland by Application of High-throughput Sequencing Technology in Sanjiang Plain

利用黑龙江省科学院自然与生态研究所三江平原野外实验研究站内3个不同小叶章生态类型湿地土壤样品,直接提取土壤微生物总DNA,应用Miseq测序技术对16S rDNA进行序列测定和分析。结果表明:不同小叶章湿地土壤细菌群落结构发生了显著变化,土壤细菌多样性和丰富度随着土壤含水率的增加而降低。草甸化湿地和沼泽化草甸湿地优势种群为酸杆菌,变形菌次之;沼泽化湿地优势种群为变形菌,酸杆菌次之。土壤含水率的增加减少了酸杆菌的分布,而增加了变形菌的分布。16S rDNAheatmap分析则表明,湿地水位的变化对酸杆菌和变形菌的群落结构影响最大。

Wetland soil bacterial community structure was studied through 16S rDNA gene. The soil samples were collected from the three different ecological types of Deyeuxia angustifolia in the Sanjiang Plain field experiment station of the institute of natural ecology, Heilongjiang Academy Sciences. Total microbial DNA was directly extracted from three soil samples, and the 16S rDNA genes were sequenced and analyzed by Miseq molecular technology. The results showed that the bacterial community composition was changed significantly, both Shannon index and Chao＇s index decreased indicating bacterial diversity was decreased with increase in soil moisture content. Acidobacteria was predominated both in meadow wetland and paludification meadows wetland, followed by Proteobacteria. The Proteobacteria was predominated in paludification wetland, followed by Acidobacteria. The distribution of Acidobacteria was decreased and Proteobacteria increased with increasing soil moisture content. The heatmap tree based on the 16S rDNA sequences showed that wetland water level affected the structure of Acidobacteria and Proteobacteria greatly.