SFRP5 mRNA在大鼠肌肉挫伤后的表达

Expression of SFRP5 m RNA in Rat Skeletal Muscle after Contusion

目的通过real-time PCR技术检测大鼠肌肉挫伤后组织中分泌型卷曲相关蛋白5(secreted frizzledrelated protein 5,SFRP5)m RNA的表达变化情况,分析其与挫伤时间及损伤类型的关系。方法 90只SD大鼠,随机分为肌肉挫伤后4、8、12、16、20、24、28、32、36、40、44、48 h组,切创后4、8 h组和对照组。于相应时间点取损伤处肌肉组织样本。提取组织中总RNA,反转录后用于SFRP5 m RNA表达量的检测。结果SFRP5 m RNA的表达量在损伤后很快明显下降,在20 h时降至最低,然后逐渐上升,但挫伤后48 h内其表达量均低于对照组;切创组SFRP5 m RNA的表达量均明显降低,在伤后4 h,切创组低于挫伤组,而在8 h时两组的表达差异无统计学意义。结论 SFRP5 m RNA在大鼠肌肉挫伤后一段时间内呈现规律性表达,可望作为推断骨骼肌损伤时间的指标之一。

Objective To investigate the relationship between the expression of secreted frizzled-related protein 5 （SFRP5） mRNA and the time interval after skeletal muscle injury in rats by real-time PCR. Methods A total of ninety SD rats were randomly divided into the contusion groups at different times including 4h, 8h, 12h, 16h, 20h, 24h, 28h, 32h, 36h, 40h, 44h, 48h after contusion, incision groups at different times including 4h and 8h after incision and the control group. The samples were taken from the contused zone at different time points. The total RNA was isolated from the samples and re- versely transcribed to analyze the expression levels of SFRP5 mRNA. Results Compared to the control group, the expression of SFRP5 mRNA in contusion groups were down-regulated within 48 h after con- tusion and reached the lowest level at 20 h, and the expression of SFRP5 mRNA gradually increased from 20 h to 48 h after contusion. The expression of SFRP5 mRNA in the incised groups were signifi- cantly lower than that of the contusion groups at 4 h after injury. At the time of 8 h, the expression levels between the contusion and incision groups showed no statistically significant difference. Conclusion It is suggested that SFRP5 mRNA analysis may show regular expression and can be a marker for estimation of skeletal muscle injury age.