钒取代的多酸盐对酪氨酸酶抑制机理的研究

The inhibitory mechanism research of vanadate-substituted polyoxometalates on tyrosinase

合成了2种不同的钒取代Keggin型多金属氧酸盐(Na4PMo11VO40和(HGly)4PMo11VO40,以下分别简写为PMo11V和Gly-PMo11V),并用紫外光谱和红外光谱对其进行结构表征.以这2种化合物为效应物,采用酶动力学方法研究其对酪氨酸酶二酚酶的抑制效果、抑制机理和抑制类型.结果表明:PMo11V和Gly-PMo11V对酪氨酸酶均有明显的抑制效果,其IC50分别为0.522和0.447mmol/L.其中,PMo11V对酪氨酸酶的抑制过程属可逆的竞争型抑制,抑制常数KI为2.629mmol/L,而Gly-PMo11V对酪氨酸酶的抑制属不可逆的抑制.综合比较,Gly-PMo11V对蘑菇酪氨酸酶的抑制效果优于PMo11V.

Two different kinds of vanadate-substituted Keggin-type polyoxometalates（Na4PMo11VO40and（HGly）4PMo11VO40,abbreviated as PMo11 V and Gly-PMo11V）were synthesized and their structures were characterized by UV and IR spectroscopy.The inhibition effects,inhibitory mechanisms and types of two effectors on mushroom tyrosinase were determined by enzyme kinetics method.Results showed that PMo11 V and Gly-PMo11 V had obvious inhibitory effects on tyrosinase with IC50 values were 0.522 and 0.447mmol/L,respectively.Moreover,PMo11 V was suggested to be a reversible and competitive inhibitor of tyrosinase with KIwas 2.629mmol/L,and Gly-PMo11 V was found to be an irreversible inhibitor of tyrosinase.Comprehensive consideration,Gly-PMo11 V was more effective against mushroom tyrosinase than PMo11 V.