康氏木霉固体发酵木聚糖酶条件的研究

Solid-state fermentation conditions for xylanase production by Trichoderma koningii

通过固体发酵培养,经单因素及正交试验分析,得出康氏木霉发酵产木聚糖酶最优条件组合为：麸皮与玉米芯质量比为2：8,硫酸铵2.5％,MnSO4 0.50％,料水比1：1.5 （g：mL）,培养基初始pH自然,培养温度30℃,发酵时间6d.在此条件下,康氏木霉固体发酵木聚糖酶活力达11.98 IU/g.该木聚糖酶水解产物富含2-5个木糖分子的低聚木糖.

By solid-state fermentation, the optimal fermentation process for xylanase production by Trichoderma koningii was investigated by single factor experiments and orthogonal experiments as follows：bran to corncob mass ratio 2：8 as carbon sources, （NH4）2SO4 2.5% as nitrogen source, MnSO4 0.25%, solid to liquid ratio 1：1.5 （g：ml）, initial pH in natural state, temperature 30 ℃ and time 6 d. Under this condition, the activity of xylanase by T. koningii reached 11.98 IU/g. The products of xylanase hydrolysis were rich in fimctional polymeric xylose bonded with 2-5 xylose molecular.