miR129在胃腺癌中的表达及意义

Expression and clinical significance of miR129 in gastric adenocarcinoma

目的探讨microRNA129(miR129)在胃管状腺瘤、胃癌组织及其对应的癌旁组织中的表达及其临床意义。方法收集经高年资病理医师确诊的55例管状腺瘤、76例胃腺癌组织及对应的癌旁组织,分别用原位杂交方法检测miR129的表达,分析miR129表达与临床病理参数之间的关系。结果癌旁胃黏膜组织中miR-129的表达阳性率高于胃管状腺瘤组织,胃管状腺瘤组织中miR-129的表达阳性率高于胃腺癌组织(P<0.05)。miR129在高分化胃癌组织中阳性表达率高于中、低分化组织(P<0.05);临床分期Ⅰ期中的阳性表达率高于Ⅲ期;无淋巴结转移组织中的表达阳性率高于有淋巴结转移组织(P<0.05);胃管状腺癌中miR129阳性率高于弥漫型腺癌(P<0.05)。结论miR129在胃腺癌组织内低表达,发挥着抑癌基因的作用;miR129可作为胃腺癌的判断预后的标志物。

Objective To investigate the expression and clinical significance of microRNA129 （ miR129 ） in gastric tubular adenoma tissues,gastric adenocarcinoma tissues and corresponding para-carcinoma tissues.Methods Fifty-five cases of gastric tubular adenoma, 76 cases of gastric adenocarcinoma and corresponding para-carcinoma tissues that were identified by senior pathology doctors were enrolled in the study.The expression levels of miR-129 were detected by in situ hybrization, and the correlation between miR129 expression and clinicopathological parameters was analyzed.Results The positive expression rate of miR-129 in para-carcinoma gastric mucosa tissues was significantly higher than that in gastric tubular adenoma tissues, moreover, which in gastric tubular adenoma tissues was significantly higher than that in gastric adenocarcinoma tissues （ P 〈0.05）.The positive expression rate in well differentiated gastric cancer tissues was significantly higher than that in moderately differentiated and poorly differentiated tissues （ P 〈0.05）.The positive expression rate of miR-129 in stageⅠcarcinoma tissues was obviously higher than that in stage Ⅲ carcinoma tissues, furthermore, which in carcinoma tissues without lymphonode metastasis was significantly higher than that in carcinoma tissues with lymphonode metastasis （ P 〈0.05）.The positive expression rate of miR-129 in gastric tubular adenoma was significantly higher than that in diffuse type adenocarcinoma （ P 〈0.05）.Conclusion The expression levels of miR129 are down-regulated in gastric adenocarcinoma,which may play a role of anti-oncogene, thus miR-129 may be regarded as a marker for evaluating the prognosis of patients with gastric adenocarcinoma.