山羊硬脊膜的微观形貌及三维结构特征

Morphology and Three-dimensional Structure Characteristics of Goat Spinal Dural

目的获取山羊硬脊膜形貌及三维微观结构特征,为构建与之空间结构近似的人工硬脊膜提供相关资料。方法山羊硬脊膜取材后,进行标本处理及大体观察,光镜、扫描电镜及透射电镜观察微观结构、组织学成分、内外表面形貌、胶原纤维直径、分布以及分布模型。结果大体观察可见硬脊膜为一乳白色、质韧、弹性半透明膜;光镜下观察硬脊膜的组成主要由"取向性"结构的胶原纤维及少量散在分布的成纤维细胞组成;扫描电镜观察显示,其内外表面如连绵山峰状隆起;透射电镜见胶原纤维有序分布,胶原纤维的平均直径为(627±60)nm,且呈现双峰分布。结论山羊硬脊膜是主要由纳米级的"取向性"胶原纤维及散在分布的成纤维细胞组成的半透明薄膜。

Objective To obtain the morphology and three-dimensional structure characteristics of goat spinal dural and provide information for reconstructing artificial spinal dural. Methods The specimens of spinal dural were obtained to observe the microstructure, surface morphology, histology, collagen fiber diameter, distribution and distribution model through naked eyes, light microscope, scanning electron microscope （SEM） and transmission electron microscope （TEM）. Results The spinal dura looked like a milky white, translucent, flexible and elastic membrane. It was mainly composed of oriented collagen fibers while fibroblasts line sparsely along those fibers. Its inner and outer surfaces looked like mountain peaks through SEM observation. It was revealed by TEM that collagen fibers distribute orderly with an average diameter of （627±60） nm. The diameters of collagen fibers exhibited a bimodal distribution. Conclusion The goat spinal dura is a translucent membrane which is mainly composed of nanoscale oriented collagen fibers and sparsely scattered fibroblasts.