Th1/Th2型细胞因子在复发性流产患者中的表达及意义

Expression of Th1/Th2 cytokines in unexplained recurrent spontaneous abortion

目的分析原因不明复发性流产(unexplained recurrent spontaneous abortion,URSA)患者外周血中Th1/Th2细胞的表达,探讨免疫因子平衡失调在URSA发病机制中的作用。方法 70例URSA患者为URSA组,正常早孕者70例为早孕组,正常未孕者70例为未孕组,采用ELISA法测定3组血白细胞介素(interleukin,IL)-2、干扰素(interferon,IFN)-α、IFN-γ以及IL-4、IL-10表达水平;以未孕组细胞因子表达水平为标准值,对URSA组和早孕组细胞因子变化值进行统计学分析。结果 URSA组IL-2[(8.37±0.57)μg/L]、IFN-α[(31.57±1.06)μg/L)、IFN-γ[(694.15±43.71)μg/L]高于早孕组[(4.18±0.49)、(5.41±0.91)、(506.19±58.23)μg/L]及未孕组[(3.98±0.61)、(5.78±0.83)、(468.39±41.58)μg/L),IL-4[(11.73±2.11)μg/L]、IL-10[(16.86±1.36)μg/L]低于早孕组[(26.18±3.68)、(28.16±4.16)μg/L]及未孕组[(25.39±2.81)、(27.72±3.48)μg/L],差异均有统计学意义(P<0.01);早孕组与未孕组各细胞因子表达水平比较差异均无统计学意义(P>0.05)。结论母体Th1细胞因子高表达、Th2细胞因子低表达与URSA发生有关,免疫平衡失调可能是介导URSA的原因之一。

Objective To analyze the expression of Th1/Th2 cells in unexplained recurrent spontaneous abortion （URSA） in peripheral blood, and discuss the function of imbalanced immune factor in the pathogenesis of URSA. Methods Seventy URSA patients （URSA group）, 70 normal early pregnant women （pregnant group） and 70 normal non pregnant women （control group） were detected interleukin （IL）-2, interferon （IFN）-α, IFN-γ, IL-4 and IL-10 in peripheral blood by ELISA method. Taking the levels of cytokines in control group as the standard values, the changes the eytokines were statistically analyzed in URSA group and pregnant group. Results The levels of IL-2 （（8. 37 ±0.57） μg/L）, IFN-α （（31.57±1. 06）μg/L） and IFN-γ （（694. 15±43. 71）μg/L） were higher in URSA group than those in pregnant group （（4.18±0.49）, （5.41±0.91）, （506.19±58.23） μg/L） and control group （（3. 98±0. 61）, （5.78±0.83）, （468. 39±41.58） μg/L）, the levels of IL-4 （（11.73±2.11）μg/L） and IL-10 （（16.86±1.36）μg/L）were lower in URSA than those in pregnant group （（26.18±3.68）, （28. 16±4.16） μg/L） and control group （（25.39±2.81）, （27. 72±3.48） μg/L） （P〈0.01）, and there were no significant differences between pregnant group and control group （P〉0.05）. Conclusion Highly expressed Th1 cytokines and lowly expressed Th2 cytokines are correlated with URSA. Imbalanced immunological function may probably be one of the causes for URSA.