七氟醚预处理对大鼠肝缺血再灌注致肺损伤的干预作用及机制探讨

Intervention of sevoflurane pretreatment on rat pulmonary injury caused by liver ischemia reperfusion and its mechanism

目的观察七氟醚预处理对肝缺血再灌注致肺损伤的干预作用,并探讨其机制。方法健康Wistar大鼠30只,随机分为假手术组、模型组、七氟醚组,每组10只。模型组与七氟醚组制作部分肝缺血再灌注模型,其中七氟醚造模前给予七氟醚吸入30 min;假手术组仅游离肝门,但不阻断血供。于再灌注60 min时处死大鼠,测血清ALT、AST、TNF-α;取部分右肺上叶组织用于测算湿重与干重比值(W/D),部分制作石蜡切片用于病理观察;取左肺组织,测定丙二醛(MDA)及MMP-9 mRNA。结果光镜下假手术组肺组织结构未见异常;七氟醚组肺组织损伤程度较模型组减轻。模型组、七氟醚组血清ALT、AST、TNF-α水平升高,与假手术组相比,P均<0.05;七氟醚组血清ALT、AST、TNF-α水平低于模型组(P均<0.05)。模型组、七氟醚组肺组织W/D、MDA含量及MMP-9 mRNA相对表达量均升高,与假手术组相比,P均<0.05;七氟醚组肺组织W/D、MDA含量及MMP-9 mRNA相对表达量低于模型组(P均<0.05)。结论七氟醚可减轻肝缺血再灌注导致的肺损伤,机制可能与降低TNF-α水平、抑制MMP-9 mRNA表达等有关。

Objective To observe the intervention of sevoflurane pretreatment on rat pulmonary injury caused by liver ischemia reperfusion and to explore its mechanism. Methods A total of 30 healthy Wistar rats were randomly divided into sham-operation group,the model group and the sevoflurane group,with 10 rats in each group. Models of liver ischemia reperfusion were established in the model group and sevoflurane group,and rats of the sevoflurane group were given inhalation of sevoflurane for 30 min before modeling; while in the sham-operation group,only porta hepatis was separated but blood supply was not blocked. Rats were scarified at 60 min after reperfusion,and the serum alanine aminotransferase（ ALT）,aspertate aminotransferase（ AST） and tumor necrosis factor-α（ TNF-α） were detected; part of right upper lobe was used to measure the wet / dry weight ratio（ W / D ratio） and part was used to make paraffin section for pathological observation; and left lung tissue was taken to determine malondialdehyde（ MDA） and MMP-9 mRNA. Results Lung tissue of the sham-operation group was normal in structure under microscopy,while damage degree of lung tissue in the sevoflurane group was reduced as compared with that of the model group. Serum ALT,AST and TNF-α of the model group and sevoflurane group were increased as compared with those of the shan-operation group（ all P〈0. 05）; while serum ALT,AST and TNF-α of the sevoflurane group was lower than that of the model group（ all P〈0. 05）. W / D ratio,MDA content and relative expression of MMP-9 mRNA in the lung tissues of the model group and sevoflurane group were increased as compared with those of the sham-operation group（ all P〈0. 05）; W / D ratio,MDA content and relative expression of MMP-9 mRNA in the lung tissues of the sevoflurane group were lower than those of the model group（ all P〈0. 05）. Conclusion Sevoflurane can alleviate the pulmonary injury caused by liver ischemia reperfusion,whose mechanism may be related to the lower levels of TNF-α and inhibition of MMP-9 mRNA expression,etc.