MCM5和P16(INK4A)在宫颈鳞癌组织中的表达及其临床意义

Expressions of MCM5 and P16^(INK4A) in cervical squamous cell carcinoma

目的探讨微小染色体维持蛋白-5(MCM5)与P16INK4A在宫颈癌组织、宫颈上皮内瘤变和正常宫颈组织中的表达及其意义。方法采用实时荧光定量PCR(Real-time PCR)和免疫组化法,分别检测MCM5和P16INK4AmRNA及蛋白在40例宫颈癌组织、11例低度宫颈上皮内瘤变(CINⅠ)、15例高度宫颈上皮内瘤变(CINⅡ~Ⅲ)和15例正常宫颈组织中的表达。结果 1各不同宫颈组织中MCM5和P16INK4AmRNA及蛋白的表达趋势一致。宫颈癌组织中MCM5和P16INK4AmRNA的表达量和蛋白表达率均高于CINⅠ、CINⅡ~Ⅲ和正常宫颈组织。MCM5基因和蛋白在宫颈癌组织中的表达均比正常宫颈组织、CINⅠ、CINⅡ~Ⅲ高,差异均具有统计学意义(P<0.05);P16INK4A基因和蛋白在宫颈癌中的表达比正常宫颈组织、CINⅠ高,差异均有统计学意义(P<0.01),而与CINⅡ~Ⅲ间差异无统计学意义(P>0.05)。2MCM5mRNA表达量和蛋白表达率与临床期别、分化程度相关,差异有统计学意义(P<0.01),与年龄无关(P>0.05);P16INK4AmRNA和蛋白表达与临床期别、年龄无关(P>0.05),而与分化程度相关,差异有统计学意义(P<0.01)。3在宫颈癌组织中MCM5和P16INK4A表达呈正相关(r=0.538,P<0.01)。结论MCM5和P16INK4A的高表达可能与宫颈癌的发生发展有关。MCM5可能成为宫颈癌肿瘤增生的新标志物;其与P16INK4A联合检测有助于进行CIN的分级和转归判断,有望提高宫颈癌的筛查率。

Objective To investigate the expressions of minichromosome maintenance protein5（MCM5）and P16INK4 A gene and protein in cervical squamous cell cancer（SCC）,cervical intraepithelial neoplasia（CIN）and normal tissues.Methods Real-time PCR and immunohistochemistry were used respectively to detect the expression levels of mRNA and protein of MCM5 and P16INK4 Ain 40 cases of cervical cancer,11 cases of CIN I,15 cases of CIN Ⅱ- Ⅲ and 15 cases of normal cervical tissues.Results 1 The expression levels of mRNA and protein of MCM5 and P16INK4 A in tissues of cervical squamous cell cancer were significantly higher than those in normal cervical tissues,CIN I and CIN Ⅱ-Ⅲtissues（P〈0.05）.The expression level of P16INK4 A was significantly higher in tissues of cervical squamous cell cancer than in normal cervical tissues and CIN I tissues（P〈0.01）.However,it did not differ from that in CIN Ⅱ-Ⅲ tissues（P〉0.05）.2 The high expression of MCM5 mRNA and protein in cervical squamous cell carcinoma was positively correlated with clinical stage and differentiation grade of cervical cancer（P〈0.01）,but not correlated with age（P〉0.05）.P16INK4 A expression exhibited no correlation with clinical stage or age of patients（P〉0.05）,but positive correlation with differentiation grade（P〈0.01）.3 A positive correlation was found between MCM5 and P16INK4 A expressions in cervical cancer（r=0.538,P〈0.01）.Conclusion The over-expressions of MCM5 and P16INK4 A may play an important role in the carcinogenesis and progression of cervical cancer.MCM5 may be used as a new marker of the proliferation of cervical cancer.Detection of P16INK4 A and MCM5 is of great significance to studying the grade and outcome of CIN,which may improve the rate of cervical cancer screening.