2种外周血涂片血小板估测方法的评价

Evaluation of 2 platelet estimation methods using peripheral blood smear

目的探讨2种外周血涂片血小板估测方法在血小板减少标本中的临床应用价值。方法收集227份乙二胺四乙酸二钾(EDTA-K2)抗凝静脉血标本,采用Sysmex XE-2100全自动血液分析仪进行血小板计数,同时采用Sysmex SP1000i自动血液制片仪制作血涂片并染色,使用血涂片A、B方法进行血小板结果估测。2种血涂片法与血液分析仪法结果进行相关性与Bland-Altman一致性分析。结果血涂片A、B方法与血液分析仪法之间相关良好,相关系数分别为0.894、0.867,P<0.000 1;Bland-Altman分析表明2种方法与血液分析仪法无系统偏差,对血小板检测结果具有较好的一致性。结论对血小板低值标本,血涂片法间接进行血小板估测具有直观、简便,且与血细胞分析法的结果相关性及一致性均较好,可用于血液分析仪血小板结果的复核,有一定的临床应用价值。

Objective To evaluate the clinical significance of 2 platelet estimation methods using peripheral blood smear in thrombocytopenia patients. Methods A total of 227 ethylene diamine tetraacetic acid-K2（ EDTA-K2）anticoagulant blood samples were collected and performed by Sysmex XE-2100 automatic hematology analyzer. The blood smears were completed and stained by Sysmex SP1000 i automatic blood smear instrument. Method A and B were used to estimate platelet count. The results of 2 platelet estimation methods were compared,and the correlations and BlandAltman consistency analysis were performed. Results Platelet counts from Method A and B showed correlation coefficients of 0. 894 and 0. 867 when compared with automatic hematology analyzer（ P 〈 0. 000 1）. Bland-Altman analysis showed that 2 methods and automatic hematology analyzer had no systematic bias,and it had consistency for platelet count.Conclusions Blood smear platelet estimation shows good correlation and consistency with automatic hematology analyzer.It is useful for the verification of the low platelet results of automatic hematology analyzer in clinical practice.