摘要
目的 观察七氟醚对老龄大鼠海马CA3区突触结构的影响.方法 健康SD大鼠60 只,18 月龄,随机分为4组:空白对照组(C组)、3%七氟醚组(S1 组)、1.5%七氟醚组(S2 组)、3%异氟醚组(Ⅰ组),每组15只.于麻醉结束后1、3、7 d ( T1 -T3 )行Morris水迷宫实验,并测定海马CA3区突触结构各指标.结果 与C组比较,Ⅰ组T1 -T3、S1 组T1 -T2 时逃避潜伏期延长,穿越原平台象限次数减少,Ⅰ组、S1 组T1 -T3 海马CA3区突触间隙增宽,PSD厚度变薄,突触活性区长度缩短,突触界面曲率减小( P 〈0.05 );与 Ⅰ 组、S1 组比较,S2 组T1 -T2时逃避潜伏期缩短,T1 -T3 海马CA3区突触间隙变窄,PSD厚度增厚,突触活性区长度延长,突触界面曲率增大(P〈0.05);与I组比较,S1 组、S2 组T3 时逃避潜伏期缩短,穿越原平台象限次数增加(P〈0.05);与S1 组比较,I组T3 时逃避潜伏期延长,穿越原平台象限次数减少(P〈0.05);与T1 比较,Ⅰ组、S1 组、S2 组T2 -T3 时逃避潜伏期缩短(P〈0.05).结论 低浓度七氟醚对老龄大鼠认知功能无明显影响,高浓度七氟醚、异氟醚均可导致老龄大鼠认知功能减退,其机制可能与抑制海马CA3区突触结构的改变有关.
Objective To observe the effects of sevoflurane on the synaptic interface structure in hippocampal CA3 area in senile rats.Methods Sixty healthy SD rats aged 18 months were randomly divided into 4 groups:blank control group(group C,n=15),3% sevoflurance group (group S1,n=15),1.5% sevoflurance group (group S2,n=15),3% isoflurane group (group Ⅰ,n=15).The Morris water maze test was performed at the 1st,3rd and 7th day ( T1 -T3 ) after anesthesia,and the synaptic structure in hippocampal CA3 area was measured.Results Compared with group C,the escape latency was longer,the frequency of crossing the original platform was reduced at T1 -T3 in group Ⅰ and at T1 -T2 in group S1,the width of synaptic cleft increased,the thickness of the postsynaptic density de-creased,the length of active zones was shortened,and the curvature of the synaptic interface decreased at T1 -T3 in group Ⅰ and group S1(P〈0.05);Compared with group Ⅰ and group S1,the escape latency was shortened at T1 -T2 in group S2,the width of synaptic cleft decreased,the thickness of the postsynaptic density increased,the length of active zones was prolonged,and the curvature of the synaptic interface was increased at T1 -T3 in group S2(P〈0.05);Com-pared with group Ⅰ,the escape latency was shortened,the frequency of crossing the original platform increased at T3 in group S1 and group S2(P〈0.05);Compared with group S1,the escape latency was longer,the frequency of crossing the original platform was reduced at T3 in group Ⅰ (P〈0.05);Compared with T1,the escape latency was shortened at T2 -T3 in group Ⅰ,group S1 and group S2(P〈0.05).Conclusion Sevoflurane of low concentration has no obvious effect on cognitive function in senile rats.Both sevoflurane and isoflurane of high concentration can induce the cogni-tive hypofunction in senile rats,and the mechanism may be associated with the inhibition of the changes of synaptic structure in hippocampal CA3 area.
出处
《实用药物与临床》
CAS
2015年第10期1143-1146,共4页
Practical Pharmacy and Clinical Remedies
基金
宁夏回族自治区自然科学基金(NZ10126)
关键词
七氟醚
认知障碍
海马
突触
异氟醚
Sevoflurane
Cognition disorders
Hippocampus
Synapses
Isoflurane