龙石斛种子萌发及原球茎增殖的研究

Study on Seed Germination and PLB Proliferation of Dendrobium Draconis

应用L9(34)正交试验设计研究不同基本培养基、不同浓度外源激素及不同浓度外源添加物香蕉汁对龙石斛种子萌发及原球茎增殖的影响,利用SPSS软件分析进行方差分析。结果表明,龙石斛种子萌发的最佳培养基是1/2MS+NAA 0.5mg/L,最适合原球茎增殖的培养基为1/2MS+NAA 0.5mg/L,6-BA 1.5mg/L+香蕉汁150g/L。

The methods of orthogonal L9（34）design was used to study the effect on the seed germination and PLB proliferation of Dendrobium Draconis of different factors,such as basic culture medium,plant hormones and banana juices.The experimental results obtained by analysis of Variance using SPSS software showed,the best culture medium for seed germination of Dendrobium Draconis was1/2MS culture medium containing NAA 0.5mg/L,and the most suitable culture medium for PLB proliferation of Dendrobium Draconis was 1/2MS culture medium containing NAA 0.5mg/L,6-BA1.5mg/L and banana juices 150g/L.