乳杆菌产生的抑酵母活性物质抑菌机理的研究

Study on inhibiting mechanism of antiyeast substances produced by Lactobacillus

以具有抑酵母活性的乳杆菌ALAC-3和ALAC-4为研究对象,以白假丝酵母为指示菌,通过测定抑菌物质粗提液对酵母菌生长曲线、细胞膜通透性、磷代谢和蛋白质表达的影响,从而初步阐明乳杆菌产生的活性物质抑酵母菌的作用机理。研究结果表明:乳杆菌产生的抑酵母活性物质对酵母菌生长曲线、细胞膜通透性、磷代谢和蛋白质表达均有影响,导致酵母菌无法正常进行生长和繁殖,从而产生抑菌作用。且ALAC-4产生的抑菌物质的抑菌性更强。

In the test, two strains of Lactobacillus ALAC-3 and ALAC-4 being of strong antiyeast ability were studied. Taking Candida albi- cans as the indicator strain, the inhibiting mechanism was explained by determining the effect of the active material on the growth curve, membrane permeability, phosphorus metabolism and protein expression of yeast. The results showed that the antiyeast substance produced by lactobacillus had the effect on the growth curve, membrane permeability, phosphorus metabolism and protein expression of yeast. So yeast can-not grow and multiply normally. And the substances coming from ALAC-4 had a stronger inhibiting yeast activity.