反硝化细菌固定化降解亚硝酸盐的研究

Research on removal of nitrite by the immobilized denitrifying bacteria

研究了以海藻酸钠(SA)、聚乙烯醇(PVA)、聚乙烯醇和海藻酸钠的混合物(PVA+SA)为包埋材料固定化反硝化细菌降解亚硝酸盐的影响,并详细考察了PVA+SA固定化反硝化菌降解亚硝酸盐的影响因素。结果表明,以PVA+SA为包埋材料制备的反硝化细菌小球与SA和PVA相比具有明显优势。较优化的条件为:PVA和SA比例10∶1,温度28℃,以葡萄糖为碳源,碳氮比2.5∶1。在较优化条件下,亚硝酸盐氮的最大去除率在72 h内能达到92%。

The effects of three kinds of immobilization methods on the removal of nitrite by denitrifying bacteria were studied including using the sodium alginate（ SA）,polyvinyl alcohol（ PVA）,polyvinyl alcohol and sodium alginate mixture（ PVA ＋ SA） for pack material respectively. At the same time,influence factors of removal of nitrite were investigated in detail for the immobilized denitrifying bacteria balls made by polyvinyl alcohol and sodium alginate mixture. The results showed that the balls of immobilized denitrifying bacteria made by polyvinyl alcohol and sodium alginate mixture had obvious advantages compared with balls made by polyvinyl alcohol and sodium alginate single. The optimal conditions were ratio of polyvinyl alcohol to sodium alginate 10∶ 1,28 ℃,with the glucose as carbon source and ratio of carbon to nitrogen 2. 5∶ 1. Under the optimal conditions,maximum removal rate of nitrite nitrogen could reach 92% in72 h.