杏鲍菇漆酶的诱导及其对染料的脱色降解

Induction Production of Laccase from Pleurotus eryngii and Dye Decolorization by the Laccase

为了研究培养条件对杏鲍菇诱导漆酶的影响，进一步开发杏鲍菇漆酶在环境治理中的应用，采用碳源、氮源、不同浓度的Cu2＋、香兰素、愈创木酚等诱导物对杏鲍菇产漆酶进行诱导并运用紫外分光光度法探索了反应温度、pH值、染料浓度和杏鲍菇漆酶用量对3种染料（酸性铬兰K、溴酚蓝、龙胆紫）的降解效果。结果表明：碳源为麦芽糖，氮源为蛋白胨时杏鲍菇产漆酶活力最高，在一定的浓度范围内，不同诱导物能促进漆酶的合成，当Cu2＋0．2mmol／L、愈创木酚0．01mmol／L、香兰素0．1～0．5mmol／L时对杏鲍菇漆酶的诱导效果最显著。杏鲍菇漆酶对染料具有较好的脱色效果，在pH值5．0、40oC条件下反应16h，酸性铬兰K、溴酚蓝、龙胆紫的脱色率分别达到76．9％、69．1％、63．8％，显示杏鲍菇漆酶在处理染料废水方面具有良好的应用前景。

In order to study the effect of culture conditions on induction of laccase from Pleurotus eryngii and explore the applieation of laccasc in environmental governance, the inductive effects of carbon and nitrogen sources, different eoneentrations of copper ions, vanillin and guaiacol on production of laccase from Pleurotus eryngii were studied, and the effect of reaction temperature, pH value, dye concentration and laccase amount on decolorization of acid chrome blue K, bromophenol blue and gentian violet were tested with the method of UV speetrophotometry. The results showed that the laccase activity of Pleurotus eryngii reached its maximmn when maltose and peptone were used as carbon source and nitrogen source. The inducer in a concentration range could promote laccase production, the hightest activity of laccase from Pleurotus eryngii was observsed at 0.2 mmol/L Cu2＋ ,0.01 mmol/L guaiaeol and 0.1--0.5 mmol/L vanillin. The laccase from Pleurotus eryngii had a good effect on decolorizing dye. Under the condition of pH value of 5.0 and temperature of 40 ℃ for 16 h,76.9% ,69.1% ,63.8% of colors were decolored for acid chrome blue K,bromophenol blue and gentian violet,respectively. A practical way using the laccase of Pleurotus eryngii to decolorize dye waste water had a good application prospect.