依诺沙星-铕络合物为探针的荧光光度法测定肝素

Spectrofluorimetric Determination on Heparin Using Enoxacin-Europium Probe

在p H值为7.40的HMT-HCl缓冲溶液中,铕（Eu3＋）能和依诺沙星（Enoxacin）形成络合物,发出Eu3＋的特征荧光,最大激发和发射波长分别为330 nm和612 nm,狭缝均为5 nm,肝素的加入能使Eu3＋的特征荧光显著增强。在线性范围0.117-5.85？g·m L-1内,肝素的浓度和荧光强度成很好的线性关系,线性方程为△F=-5.691＋6.98 c（r=0.994）（c：μg·m L-1）;检出限为0.06μg·m L-1。本方法用于实际样品的测定,灵敏度高、选择性好、干扰少、操作简单,结果令人满意。

A new method for determination of Heparin by fluorometry method was established. In HMT-HCl buffer solution at p H 7.40, Eu3＋ formed complex with enoxacin, which emitted characteristic fluorescence of Eu3＋ with maximum excitation wavelength at 330 nm, maximum emission wavelength at 612 nm and slit width at 5 nm. Additional Heparin resulted in a remarkable increase in the Eu3＋ characteristic fluorescence. Within the range of 0.117＋5.85 μg·m L-1, the concentration of Heparin had a linear relationship with the fluorescent intensity, with linear equation of △F =-5.691 ＋6.98 c（r = 0.994）（c：μg·m L-1）and detection limit of 0.06 ？g·m L-1. This method generated high selectivity, high sensitivity and low disturbance,which had been applied to the determination of practical samples with satisfactory result.