大豆异黄酮与芽孢杆菌对缓解黄羽肉仔鸡肠道氧化应激的影响

Effect of Soy Isoflavones and Bacillus on Dampening Oxidative Stress of Intestine in Yellow-Feathered Broiler Chickens

为研究大豆异黄酮、芽孢杆菌对在氧化豆油作用下黄羽肉仔鸡生长性能、免疫器官及肠道抗氧化水平的影响作用,选用1日龄快大型黄羽肉仔鸡480只,随机分成4组,每组6个重复,每个重复20只鸡。对照组为玉米-豆粕型基础日粮,氧化组是将对照组中的新鲜豆油替换成氧化豆油,大豆异黄酮组是在氧化豆油组的基础上添加20 mg/kg大豆异黄酮,芽孢杆菌组是在氧化豆油组基础上添加0.1%芽孢杆菌。结果表明:大豆异黄酮、芽孢杆菌对黄羽肉仔鸡生长性能无显著影响(P>0.05)。添加0.1%芽孢杆菌对免疫器官指数有一定提高作用,但是不显著(P>0.05)。添加0.1%芽孢杆菌可以显著提高十二指肠GSH的含量,空肠GSH-Px的活性(P<0.05),同时还显著提高十二指肠ESR-α、claudin-1、occludin及回肠occludin基因的表达水平(P<0.05)。综合生长性能、免疫器官指数及肠道酶活、基因定量的结果,认为在饲粮中添加0.1%芽孢杆菌能够一定程度上缓解黄羽肉仔鸡肠道应激,保护仔鸡的肠道屏障完整。

To study the effects of soy isoflavones and bacillus on growth performance,immunity and antioxidative capacity of intestine of yellow-feathered broiler chickens using oxidized soybean oil as an oxidant,an experiment was conducted using a total of 480 one-day-old yellow-feathered broiler chickens. Chickens were randomly divided into4 treatments（30 for each treatment,4 for each replicate）. The control group（treatment 1）was fed the basal diet. The oxidant group was the same levels of all nutrients except that fresh soybean oil was replaced by oxidized soybean oil.The soy isoflavone group was the oxidant group diets added with 20 mg/kg soy isoflavone. The bacillus group was the oxidant group diets added with 0.1% bacillus. The results showed that soy isoflavone and bacillus did not significantly affect growth performance（P0.05）. 0.1% bacillus increased the weight of immune organs but not significantly（P0.05）.Oxidized diet supplement with 0.1% bacillus significantly increased the content of GSH in duodenun and the activity of GSH-Px in jejunum,while increased the expression of ESR-α,claudin-1,occludin in duodenun and the expression of occludin in ileum. The results from this study indicated that dietary supplementation with 0.1% bacillus can dampene oxidative stress of intestine and protect the mucosal barrier function intact.