华重楼植株的快速繁殖研究

Rapid propagation of Paris polyphylla var. chinensis

目的以华重楼Paris polyphylla var.chinensis根茎为外植体,建立华重楼植物的快速繁殖技术。方法以MS为基本培养基,通过研究激素6-BA、2,4-D、NAA、IBA、KT、IAA和头孢曲松钠、活性炭等对愈伤组织诱导、不定芽分化和不定根产生的影响,找出组培苗诱导培养的最佳培养基配方。结果华重楼最佳愈伤组织诱导培养基为MS+6-BA 1.0 mg/L+NAA 1.5mg/L+2,4-D 3.0 mg/L;最佳不定芽分化培养基为MS+6-BA 3.0 mg/L+IAA 0.3 mg/L+KT 1.0 mg/L+头孢曲松钠300 mg/L;最佳生根培养基为1/2 MS+IBA 0.5 mg/L+NAA 0.1 mg/L+活性炭0.5%。当组培苗长出第1片叶且不定根长出4条以上时将组培苗取出,先放在无菌水中栽培5 d,再移栽至松软土壤中,组培苗生长健壮,成活率为100%。结论在获得愈伤组织的基础上,进一步完成对不定芽的诱导与无根苗生根培养,筛选出了培育华重楼组培苗的最佳条件。

Objective The roots of Paris polyphylla var. chinensis were used as explants to establish a rapid propagation technique. Methods The effects of hormone 6-BA, 2,4-D, NAA, IBA, KT, IAA, ceftriaxone, activated carbon, etc on callus induction, differentiation of adventitious bud, and formation of adventitious roots were studied to find out the best culture medium formulation by using MS as basic culture medium. Results The best medium for callus induction of P. polyphylla var. chinensis was MS ＋ 6-BA 1.0 mg/L ＋ NAA 1.5 mg/L ＋ 2,4-D 3.0 mg/L, and the best medium for differentiation of adventitious bud was MS ＋ 6-BA 3.0 mg/L ＋ IAA 0.3 mg/L ＋ KT 1.0 mg/L ＋ ceftriaxone 300 mg/L, while the best medium for formation of adventitious roots was 1/2 MS ＋ IBA 0.5 mg/L ＋ NAA 0.1 mg/L ＋ activated carbon 0.5%. The tissue cultured seedling was moved to sterile water for 5 d when the first leaf emerged and four adventitious roots appeared, then the seedling was transplanted into the soft soil. The tissue cultured seedling would grow strongly with 100% survival rate. Conclusion The adventitious bud is inducted and then the root from bare-root seedling is cultured on the basis of obtained callus. Finally the best conditions to foster the tissue cultured seedling of P. polyphylla var. chinensis have been screened.