骨碎补化学成分研究

Studies on chemical constituents of Drynaria roosii

目的研究骨碎补（槲蕨Drynaria roosii的干燥根茎）的化学成分。方法采用溶剂法提取,再利用硅胶、ODS、Sephadex LH-20及高效制备液相等柱色谱方法进行分离纯化,通过化合物理化性质及MS、NMR等波谱技术鉴定化合物结构。结果从骨碎补75%乙醇提取物中分离得到7个化合物,分别鉴定为12-O-咖啡酰基-12-羟基正十二烷酸甲酯（1）、对羟基苯丙酸（2）、3,4-二羟基-苯乙醇8-O-β-D-吡喃阿洛糖苷（3）、木犀草素7-O-β-D-吡喃葡萄糖苷（4）、补骨脂素（5）、原儿茶酸（6）及咖啡酸（7）。结论化合物1为新化合物,命名为骨碎补烷酸酯A,2~5为首次从槲蕨属植物中分离得到。

Objective To investigate the chemical constituents from the rhizomes of Drynaria fortunei. Methods The compounds were purified by silica gel, ODS, Sephadex LH-20 column chromatography, and preparative HPLC, and their structures were identified on the basis of chemical and spectroscopic methods, such as MS and NMR. Results Seven compounds were isolated from the 75% ethanol extract and identified as 12-O-caffeoyl-12-hydroxyldodecanoic acid methyl ester（1）, p-hydroxy benzenepropanoic acid（2）, 3,4-dihydroxyphenyl ethyl 8-O-β-D-allopyranoside（3）, luteolin 7-O-β-D-glucopyranoside（4）, psoralen（5）, protocatechoic acid（6）, and caffeic acid（7）. Conclusion Compound 1 is a new compound, named drynalkylester A, and compounds 2—5 are obtained from the plants of Drynaria Willd. for the first time.