微小RNA-125b及其靶基因信号素分子4C在乳腺癌中的表达及意义

Expressions of microRNA-125b and semaphorin 4C in breast cancer and its significance

目的：探讨微小RNA（microRNA,miR）-125b及其靶基因信号素分子（SEMA）4C在乳腺癌组织（BRCR）及其癌旁正常组织（NCT）中的表达情况,并分析其与临床病理学特征的关联性及意义.方法：采用荧光定量PCR检测24 例BRCR及其NCT中miR-125b的表达,采用免疫组织化学方法检测40例BRCR及其NCT中SEMA4C的表达.统计分析两者表达水平与患者的年龄、组织学分级、临床分期、淋巴结转移,雌、孕激素受体和人表皮生长因子受体-2（cerbB-2）等临床病理学特征间的关联性.结果：SEMA4C表达在BRCR中高于NCT,SEMA4C表达在淋巴结转移和cerbB-2表达间差异均有统计学意义（P〈0.01和P〈0.05）,而在患者年龄,组织学分级,肿瘤临床分期及雌、孕激素受体表达间差异均无统计学意义（P〉0.05）.miR-125b表达在BRCR中低于NCT（P〈0.05）,其表达在SEMA4C、淋巴结转移以及cerbB-2差异均有统计学意义（P=0.034 -P=0.022）.结论：在乳腺浸润性导管癌中SEMA4C表达升高,miR-125b表达降低,两者均与cerbB-2过表达以及淋巴结转移均有一定关系,提示SEMA4C为miR-125b靶基因之一,miR-125b可能是一个新的乳腺癌标志物.

Objective：To explore the expressions of microRNA（miR）-125b and its target gene semaphorin4C（SEMA4C） in breast cancer tissue（BRCR） and adjacent normal tissue（NCT）,and analyze its correlation with clinicopathologic features and significance. Methods：The expressions of miR-125b in 24 BRCR and NCT were detected using real-time quantitative PCR,and the expressions of SEMA4C in 40 BRCR and NCT were detected by immunohistochemistry. The relationships between the expressions of miR-125b and SEMA4C and clinico-pathological parameters including age,clinical staging,lymph node metastasis,estrogen receptor,progesterone and human epidermal growth factor receptor-2（cerbB-2） were analyzed. Results：The expression of SEMA4C in BRCR was higher than that in NCT,the difference between the expression of SEMA4C and cerbB-2 in lymph node metastasis tissue was statistically significant（P〈0. 01 and P〈0. 05）. The differences of the age,histological grading,clinical staging,and estrogen and progesterone receptors were not statistically significant（P〉0. 05）. The expression of miR-125b in BRCR was lower than that in NCT（P〈0. 05）,the differences between the expression of SEMA4C and cerbB-2 in lymph node metastasis tissue were statistically significant （ P =0. 034 to P =0. 022）. Conclusions：The expressions of miR-125b and SEMA4C in breast invasive ductal carcinoma increase and decrease, respectively,which is associated with lymph node metastasis and cerbB-2 level. The SEMA4C is one target gene of miR-125b,and miR-125b may serve as a potential breast cancer marker.