摘要
以3个不同小麦品种中国春、宁春4号和宁春42号为材料,在N、P2O5、K2O不同营养水平下进行栽培(盆栽)试验,利用SDS-PAGE电泳等分析技术,研究肥力营养对麦谷蛋白和HMW-GS亚基动态形成的影响。结果表明:(1)在不同水平施肥处理下各品种开花后谷蛋白形成时间不尽相同,但开花后麦谷蛋白积累变化均呈"低—高—低"总趋势,供试3个品种随籽粒发育成熟谷蛋白含量逐渐增多,大约在开花后25d总含量达最大积累,之后稍有下降;(2)在不同施肥处理水平下3个品种高分子量谷蛋白亚基花后5 d开始至15 d全部形成,且品种不同亚基形成时间不同,同一品种内亚基形成顺序不同,以Glu-D1x和Glu-B1x位点编码的亚基最早出现,Glu-A1编码的亚基形成最晚;(3)氮、磷、钾肥影响供试品种宁春4号、宁春42号高分子量谷蛋白亚基的组成。
In order to provide theoretical basis for high quality cultivation of wheat, with 3 different wheat varieties, including China spring, Ningchun NO.4 and Ningchun N0.42 as materials, under different timing and stages of nitrogen, phosphorus, potassium application, pot experiments were carried out to study the composition and expression of High Molecular Weight Glutenin Subunit （ HMW-GS ） . The results showed that, in different fertilization treatments, Glutenin formed after flowering time differently, the general trend of Glutenin accumulation changes was dynamic ＂low-high-low＂ ~ With grain growth and maturity, Glutelin contents of 3 varieties gradually increased, reached the maximum accumulation at about 25 days after flowering, then a slight decline. In different fertilizer treatments, HMW-GSs of 3 varieties all formed in 5-15 days after flowering. The subunit formation time was different in different varieties, and the forming order of HMW-GS was different in the same cultivar, the suhunits coded by Glu-Dlx and Glu-Blx formed first, while the subuuits coded by Glu-AI formed last. Nitrogen, phosphorus, potassium application had effects on composition of HMW-GS in Ningchun NO.4 and Ningchun NO.42.
出处
《广东农业科学》
CAS
2015年第19期8-13,共6页
Guangdong Agricultural Sciences
基金
银川能源学院校级科研项目(2013-KY-Z-13)
