濒危植物紫背天葵种质离体保存技术研究

Research on germplasm in vitro preservation technology of endangered plant Begonia fimbristipula

以濒危植物紫背天葵试管苗为材料,研究其种质离体保存的最佳途径。结果表明：常规培养基6-BA与NAA组合并不利于紫背天葵离体种质的保存,保存90 d后存活率仅有5%。PP333抑制紫背天葵试管苗芽丛的分化,显著促进不定根的生成,保存180 d后,仅3.0 mg/L PP333存活率达到55%,种质在恢复生长时有变异现象。低浓度ABA（0.5~2.0 mg/L）具有抑制紫背天葵试管苗生长的作用,保存180 d后,试管苗存活率均为100%,且恢复培养时组培苗长势较好。1/2 MS培养基对紫背天葵试管苗保存效果最好,保存240 d后存活率仍高达85%,经恢复生长后,试管苗的增殖及其长势均优于其他处理,可确定为紫背天葵种质离体保存的最佳方法。

Taking tube seedlings of endangered plant Begonia fimbristipula as materials, the best way of in vitro preservation of germplasm was studied. The results showed that conventional medium 6-BA and NAA combination was not suitable for germplasm in vitro preservation of B. fimbristipula, the survival rate was only 5% after 90 d. PP333 inhibited the differentiation of tube seedlings sprout plexus, significantly promoted the formation of adventitious roots, after 180 d, only the survival rate of 3.0 mg/L PP333 treatment reached 55%, variation of germplasm in restoring growth. ABA （ 0.5-2.0 mg/L ） inhibited the growth of tube seedlings, after 180 d, the test-tube seedling survival rate was 100%, the somaclone was better after recovery training. 1/2 MS culture medium had the best effect on B. fimbristipula, the survival rate was as high as 85% after 240 d. After restoring growth, the tube seedlings multiplication and their growth were superior to other processings. 1/2 MS culture medium can be used as the best training method for germplasm in vitro preservation of B.fimbristipula.