红参对支气管哮喘模型小鼠气道炎症的影响

The inhibitory effects of water extracts of Red Ginseng on airway inflamation in mouse with bronchial asthma

目的：探讨红参水提物对哮喘小鼠气道炎症的治疗作用及其机制。方法：把60只雌性BABL/c小鼠随机数字表法分为6组,每组10只,分别为正常对照组、哮喘模型组、红参水提物200 mg/kg剂量组、红参水提物100 mg/kg剂量组、红参水提物50mg/kg剂量组、地塞米松0.5 mg/kg组。在末次激发24 h后所有小鼠取左肺组织行HE染色,观察哮喘模型组肺组织炎症浸润情况,用酶联免疫吸附法（ELISA）观察小鼠支气管肺泡灌洗液（BALF）中细胞的总数和各种炎症细胞计数的变化以及白细胞介素-4（Interleukin 4,IL-4）、IL-5含量;取右肺组织用Western blot检测NF-κB p65蛋白的表达情况。结果：哮喘模型组小鼠与对照组相比较BALF中的炎症细胞计数、IL-4、IL-5水平增高,肺组织中的NF-κB p65表达水平显著高于对照组;红参水提物组有效剂量（100-200 mg/kg）和地塞米松组小鼠与哮喘组小鼠相比炎症细胞计数、IL-4、IL-5以及NF-κB p65蛋白表达水平均显著降低,具有显著的差异。结论：红参水提物在体内可能主要通过抑制NF-κB p65等核转录因子来调节Th2反应,以此来抑制气道炎症反应。

Objective： The effect and mechanism of action of effect of water extracts of Red Ginseng（ WERG） to asthma mice airway inflammation and remodeling. Methods： Preparations for the models of the asthma mouse 60 Male BALB / c mice,were randomly divided into 6 groups,and the groups were asthma group,contral group,WERG 200 mg / kg group,WERG 100 mg / kg group,WERG 50 mg / kg group,dexamethasone group. All mice were killed 24 h after final OVA challenge. The left lung was isolated for pathological examination. Lung sections were stained with hematoxylin and eosin（ HE）. The concentrations of IL-4,IL-5 in BALF were measured by ELISA,the western blote was performed to detect NF-κB p65 protein from the right lung tissues. Results： In asthma group,the number of inflammatory cells and the expression of IL-4,IL-5 in BALF is higher than the control group,NF-κB p65 in lung is significantly higher than those in control group（ P 〈0.05）. WERG（ 100 - 200 mg / kg） group,dexamethasone group significantly decreased. The number of inflammatory cells,the expression of IL-4、IL-5 in BALF,the content of NF-κB p65 proteinin lung tissues were significantly lower,but IFN-γ were higher,with differences（ P〈0. 05）. Conclusion： These findings indicate that WERG protects against OVA-induced airway inflammation,at least in part via downregulation of NF-κB activity.