乌丹降脂胶囊对大鼠非酒精性脂肪肝模型的治疗作用

Study of Danwujiangzhi capsule on treatment in rats with NAFLD

目的:探讨乌丹降脂胶囊治疗大鼠非酒精性脂肪肝(NASH)的作用及可能的治疗机制。方法:90只大鼠随机分为6组,分别为正常对照组、模型对照组、易复善组和乌丹降脂胶囊104.17 mg/kg、208.34 mg/kg、416.67 mg/kg剂量组。正常对照组以基础饲料喂食,而模型对照组、易复善组和乌丹降脂胶囊104.17 mg/kg、208.34 mg/kg、416.67 mg/kg剂量组则以高脂饲料喂饲,连续喂养16周;喂养2周后,正常对照组、模型对照组以生理盐水10ml/kg灌胃;易复善组以易复善溶液灌胃85.2mg/kg;乌丹降脂胶囊104.17 mg/kg、208.34 mg/kg、416.67 mg/kg剂量组以乌丹降脂胶囊不同浓度溶液灌胃10ml/kg,连续灌胃14周,测量大鼠体重、肝湿重、肝指数,检测血清生化指标及肝组织病理改变。结果:与模型组相比,乌丹降脂胶囊416.67 mg/kg组及易复善组体重、肝湿重及肝指数均明显降低,血液生化ALT、AST、CHOL、TG、LDL-C、CRP各指标水平明显下降,HDL-C水平明显升高;与模型组相比,病理HE染色结果显示,易复善组与乌丹降脂胶囊416.67 mg/kg组肝脏肝小叶结构恢复,肝细胞索及肝窦结构清晰,肝细胞内脂肪空泡显著减少。结论:乌丹降脂胶囊具有治疗非酒精性脂肪肝作用,机制可能与调节脂质代谢有关。

Objective： To explore the effect and treating mechanism of Danwujiangzhi capsule on experimental model of nonalcoholic fatty liver in rat. Methods： A total of 90 healthy sprague-dawle rats were randomly divided into 6 groups： control group,model control group,Yifusan group and Wudanjiangzhi capsule（ 104 mg / kg,208 mg / kg,417 mg / kg） group. Normal control group fed based forage,and the other five groups fed high fat forage,Continuously for 16 weeks. Two weeks later,the normal control group,model control group with saline 10 ml / kg.（ d） to fill the stomach,Yifusan group with Yifusan solution for 10 ml/kg.（ d） to fill the stomach. Wudanjiangzhi capsule（ 104 mg/kg,208 mg / kg,417 mg / kg group with Wudanjiangzhi capsule of different concentration solution 10 ml / kg.（ d） to fill the stomach. After 14 weeks,measuring the rat body weight,wet weight of liver and liver index,testing the serum biochemical indicators and liver tissue pathologic change. Results： Compared with model group,the body weight,liver wet weight and liver index of Wudanjiangzhi capsule 416. 67 mg / kg group and Yifusan group is reduced,and the difference was statistically significant（ P〈0. 05）. Blood biochemical ALT,AST,CHOL,TG,LDL- C,CRP level declined obviously,HDL- C level is increased,and the difference statistically significant（ P〈0. 05）. Compared with model group,the pathological HE staining results showed that Wudanjiangzhi capsule 417 mg / kg group and Yifusan group liver structure of hepatic lobule,liver cells and hepatic sinus structure clear,liver fat vacuoles in cells was significantly reduced. Conclusion： Wudanjiangzhi capsule has the effect treating nonalcoholic fatty liver disease,the mechanism may be related to the regulation of lipid metabolism.