急性脑损伤大鼠海马代谢变化的1H NMR研究

Metabolic changes detected by ~1H NMR spectroscopy in the hippocampus of acute brain injury rats

目的通过检测急性脑损伤大鼠(ABI)海马核磁共振(NMR)氢谱的改变来揭示其代谢轮廓的变化。方法 SD大鼠通过外伤造成ABI模型,收集大鼠的海马并进行NMR分析。结果与对照组大鼠相比,ABI组大鼠的海马代谢轮廓明显不同,谷氨酸(P<0.01)、牛磺酸(P<0.05)、肌醇(P<0.05)、谷氨酰胺(P<0.05)等代谢产物出现了显著变化。结论代谢组学结果表明,ABI发生时,海马内神经递质代谢出现了紊乱,这为进一步治愈ABI提供了思路。

Objective To reveal the alterations of metabolic profiles by detecting changes in hippocampus 1H NMR spectra in acute brain injury（ABI） rats. Methods The model of ABI was achieved by trauma,and 2 hours after modeling,the hippocampus samples were collected for NMR analysis. Results Compared to age-matched control rats,the ABI rats exhibited markedly different metabolic profiles,which showed significant changes in glutamate（P〈0.01）,taurine（P〈0.05）,myo-inositol（P〈0.05）,and glutamine（P〈0.05）. Conclusion The metabonomics results suggest that amino acid metabolism is disordered when ABI occurs. This study provides clues for further understanding to cure ABI.