基于光镊原理的光纤激光驱动细胞方法

Driving Method of the Cell by the Optical Fiber Laser Based on the Principle of Optical Tweezers

提出了一种利用聚焦激光通过单模光纤对酵母细胞驱动的实验方法。基于光压产生光镊的原理,在微通道宽度为100μm的微流控芯片中,波长为650 nm、功率为200 mW的红色半导体激光通过单模光纤对酵母细胞进行驱动。其中有两个关键技术对实验起决定性作用,即激光到光纤的高耦合度和在微流控芯片中以水利聚焦形成单列细胞层流。实验结果表明:在该实验条件下,聚焦激光可以通过单模光纤对酵母细胞进行驱动,且平均速度约为25μm/s,达到了一个较高的驱动速度。该实验方法具有结构简单、检测精确度高及设备成本较低等特点。

An experimental method of driving the yeast cells by focusing the laser through the single mode optical fiber was proposed.The yeast cells were driven by the 650 nm wavelength and 200 mW power red semiconductor laser through the single mode optical fiber in the microfluidic chip with the microchannel width of 100μm based on the principle forming optical tweezers by the optical pressure.There were two key technologies to be crucial for the experiment,i.e.the high coupling degree of the laser to the optical fiber and a single cell laminar flow in the microfluidic chip formed by hydrodynamic focusing.The experimental results show that under the experimental conditions,the yeast cells are driven by focusing the laser through the single mode optical fiber,and the average speed is about 25μm/s which reaches a high driving speed.The experimental method has the advantages of simple structure,high detection accuracy and low equipment cost,and so on.