黑曲霉菌株产纤维素酶的固态发酵条件优化

Optimization of Solid-state Fermentation Condition of Aspergillus niger Strain with Producing Cellulase

为提高纤维素的利用率,以麸皮、玉米芯为主要原料,采用单因素试验和L9(34)正交试验方法,分别研究碳源、氮源、表面活性剂、培养基初始pH、料水比、培养温度及时间对黑曲霉XZ-3S产纤维素酶的影响,并在此基础上研究黑曲霉XZ-3S产纤维素酶的最优固态发酵条件。结果表明:最优发酵条件为麸皮与玉米芯的比例6∶2,硫酸铵浓度4.0%,吐温-40 0.4%,发酵培养基初始pH 4.0,培养温度28℃,培养时间96h,培养基料水比1∶1.5。在该工艺条件下,CMC酶活性及FPA酶活性分别达273.56IU/g和135.61IU/g。

The effect Of different carbon source, nitrogen source, surfactant, initial pH, medium material/water ratio, temperature and time based on main raw materials of wheat bran and corn cob on cellucase activity of A. niger XZ-3S strain by single-factor and L9 （34） orthogonal test and then the solid- state fermentation condition of A. niger XZ-3S strain with producing cellulase was optimized to improve utilization efficiency of cellulose. Results： The optimal fermentation condition of A. niger XZ-3S strain with producing cellulase includes 6 ：2 of wheat bran/corn cob ratio, 4.0% ammonium sulfate, 0. 4% Twain-40, initial pH 4.0, 28℃, 96 h and 1 ： 1.5 of medium material/water ratio. The activity of CMC and FPA enzyme of A. niger XZ-3S strain with producing cellulase can reach 273.56 IU/g and 135.61 IU/ g under the optimal fermentation condition respectively.