紫外光辐照对脱氧雪腐镰刀菌烯醇和T-2毒素的去除作用

Removal of Deoxynivalenol and T-2 Toxin by Ultraviolet Irradiation

目的:研究紫外光辐照对脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)和T-2毒素的去除作用。方法:测定不同辐照时间、不同辐照距离、不同pH值条件下紫外光辐照对DON和T-2毒素影响,采用高效液相色谱-质谱联用技术测定毒素及其衍生物,外标法定量。结果:经紫外光辐照后,溶液中DON、T-2毒素的质量浓度均随着辐照时间的延长而不断减小,随着辐照距离和pH值的减小而不断减小。pH 7的1.0μg/mL DON、T-2毒素溶液,在紫外灯功率20 W、辐照距离150 mm条件下辐照60 min后,DON、T-2毒素去除率分别为(84.90±2.52)%、(74.60±2.74)%。紫外光辐照后,毒素溶液中不含有已知的毒素衍生物,可能被转化成新的未知产物。结论:在非碱性条件下,紫外光辐照对DON、T-2毒素具有明显的去除作用。

The effects of irradiation time,irradiation distance and pH of toxin solutions on the removal of deoxynivalenol（DON） and T-2 toxin by ultraviolet（UV） irradiation were investigated.The concentration of toxins and their derivatives were analyzed by high performance liquid chromatography coupled with tandem mass spectrometry（HPLC-MS/MS） and quantified by an external standard method.The concentration of DON and T-2 toxin in solutions decreased with increasing irradiation time and decreasing irradiation distance and pH of toxin solutions.The removal rates of DON and T-2 toxin were（84.90 ± 2.52）%and（74.60 ± 2.74）%after UV irradiation for 60 min under the conditions of 7,1.0 μg/mL,20 W and150 mm for toxin solution pH,toxin concentration,UV light power,and irradiation distance,respectively.The solutions containing DON and T-2 toxin did not contain known derivatives of both toxins after UV irradiation.DON and T-2 toxin might be transformed into new unknown products.DON and T-2 toxin were removed obviously by UV irradiation in non-alkaline condition.