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甜瓜细菌性果斑病病原菌的分离鉴定及16S rDNA序列分析 被引量:2

Isolation,Identification and 16S rDNA Sequence Analysis of Bacterial Fruit Blotch Pathogen in Melon
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摘要 从新疆昌吉甜瓜实验田中采集发病甜瓜样品12份,对甜瓜细菌性果斑病病原菌进行分离纯化,并根据形态观察、致病性测定得到甜瓜细菌性果斑病病原菌1株。以该菌总DNA为模板,采用细菌16S rDNA通用引物进行聚合酶链式反应扩增,并克隆到pGM-T载体,测序结果表明:克隆的16S rDNA序列长度为1 493 bp,通过GenBank上序列比对搜索工具分析,并构建系统发育树,对该菌株进行鉴定,结果表明该菌属于Acidovorax avenae subsp.citrulli Willems(Aac),即燕麦嗜酸菌西瓜亚种。 Twelve fruits of melon with bacterial fruit blotch collected from Changji,Xinjiang were investigated for the isolation and purification of the pathogen.The pathogenic strain was subjected to polymerase chain reaction(PCR) amplification with the bacterial 16 S rDNA universal primer using its total DNA as the template and the products were cloned into pGM-T vector.Sequencing analysis revealed that the fragment had a length of 1 493 bp.Basic Local Alignment Search Tool(BLAST) and phylogenetic tree analysis showed that the strain belongs to Acidovorax avenae subsp.citrulli Willems(Aac).
出处 《食品科学》 EI CAS CSCD 北大核心 2015年第19期186-189,共4页 Food Science
基金 新疆维吾尔自治区自然科学基金项目(2011211B09)
关键词 甜瓜 细菌性果斑病 燕麦嗜酸菌西瓜亚种 鉴定 melon bacterial fruit blotch Acidovorax avenae subsp.citrulli Willems identification
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