明日叶查耳酮对Ⅱ型糖尿病大鼠脂代谢的影响

Effect of Ashitaba Chalcone on Lipid Metabolism of Rats with Type II Diabetes

目的:研究明日叶查耳酮和游泳运动对Ⅱ型糖尿病大鼠脂代谢的影响。方法:注射链脲佐菌素诱导成年雄性Sprague-Dawley(SD)大鼠糖尿病模型,观察补充明日叶查耳酮、游泳运动、游泳运动结合补充明日叶查耳酮,以及阳性药物二甲双胍对糖尿病大鼠血清甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白(low density lipoprotein,LDL)、高密度脂蛋白(high density lipoprotein,HDL)、游离脂肪酸(free fatty acid,FFA)水平的影响,以及对肝脏内脂素(visfatin)mRNA、甘油-3-磷酸酰基转移酶-1(glycerol-3-phosphate acyltransferase-1,GPAT-1)mRNA、肉碱棕榈酰转移酶-1(carnitine palmitoyl transferase-1,CPT-1)mRNA相对表达量的影响。结果:与糖尿病组比较,各干预组大鼠的TG、TC、LDL、FFA水平显著下降,HDL水平显著升高;各干预组大鼠肝脏visfatin mRNA、GPAT-1 mRNA相对表达量显著下降;肝脏CPT-1 mRNA相对表达量则均显著升高。结论:明日叶查耳酮有很好的改善糖尿病大鼠脂代谢的能力,并且与游泳运动产生协同效果。

Objective：To study the effect of oral ashitaba chalcone and swimming exercise on lipid metabolism of rats with type II diabetes.Methods：Adult male streptozotocin-induced diabetic rats were subjected to swimming exercise and/or oral administration of ashitaba chalcone.Changes in the serum contents of triglyceride（TG）,total cholesterol（TC）,low density lipoprotein（LDL）,high density lipoprotein（HDL） and free fatty acid（FFA）,and the relative mRNA expression levels of visfatin,glycerol-3-phosphate acyltransferase-1（GPAT-1） and carnitine palmitoyl transferase-1（CPT-1） in liver tissue were measured.Results：Compared with the diabetic model group,the serum levels of TG,TC,LDL and FFA contents were significantly lower in the intervention groups,while the serum level of HDL was significantly increased.The mRNA expression levels of visfatin and GPAT-1 in liver were remarkably decreased in the treated diabetic groups;however,the relative mRNA expression of CPT-1 in liver mRNA was significantly enhanced.Conclusion：Ashitaba chalcones improve lipid metabolism in diabetic rats showing a synergistic effect with exercise intervention.