外源物质对番茄耐冷性相关指标的影响

Effect of exogenous substances on tomato cold tolerance index

以番茄为试验材料,在番茄幼苗四叶一心时喷施三种外源物质ABA(10 mg·L-1)、Ca Cl2(1 200 mg·L-1)、SA(300 mg·L-1),一次性喷施3 d后放入人工气候箱中低温胁迫处理(昼/夜:10℃/6℃),低温处理5 d后测定冷害指数和各项指标,研究三种外源物质对番茄幼苗耐冷性相关指标的影响。结果表明,低温胁迫后,番茄体内脯氨酸含量上升,CAT活性、叶绿体膜和类囊体膜ATP酶活性下降。喷施ABA、Ca Cl2、SA三种外源物质后,可缓解番茄幼苗冷害程度,表现为冷害指数显著下降,脯氨酸含量、CAT活性、叶绿体膜和类囊体膜ATP酶活性显著升高。其中,以喷施1 200 mg·L-1Ca C12冷害指数最低,其他指标(包括脯氨酸含量、CAT活性、叶绿体膜和类囊体膜ATP酶活性)表现最好,故效果最佳。

Tomato as test material, spraying three exogenous substances at the four-leaf and oneheart seedlings, ABA（10 mg· L-1）, Ca Cl2（1 200 mg· L-1）, SA（300 mg· L-1）, after spraying 3 d material was put into the artificial climate chamber cold stress treatment（day/night： 10 ℃ /6 ℃）, chilling injury index and the other indices were measured after low temperature for 5 d. The results showed that low temperature stress, tomato proline content increased, CAT activity, chloroplast membrane and thylakoid membrane ATP activity decreased. Spraying the three exogenous substances could ease the degree of chilling injury in tomato seedlings, and the chilling injury index decreased significantly, proline content,CAT activity, chloroplast membrane and thylakoid membrane ATP activity significantly increased.Among them, spraying Ca Cl2（1 200 mg· L-1） was the best.