温阳、益气方药对心肌梗死后心力衰竭大鼠肾素、血管紧张素Ⅱ及醛固酮时相性变化的影响

Effects of comparison of warming yang prescription with invigorating qi prescription on renin, angiotensin Ⅱ, and aldosterone at different time phase in rats with heart failure after myocardial infarction

目的:比较温阳、益气两类药物不同用药时间点对心力衰竭(简称心衰)大鼠血浆中肾素、血管紧张素Ⅱ、醛固酮含量的影响,为临床治疗慢性心衰及选择用药周期提供部分实验依据。方法:3批动物均采用冠脉结扎法造成心肌梗死后心衰动物模型,根据左心室射血分数平均分为模型组、温阳(附子、肉桂干浸膏)组、益气(人参、黄芪干浸膏)组、温阳+益气(附子、肉桂干浸膏∶人参、黄芪干浸膏=1∶1)组、卡托普利组、地高辛组,每组6只,另设假手术组。心肌梗死手术后第2天开始给予药物干预治疗,每日灌胃1次,3个批次实验大鼠分别药物干预1、2、4周后腹主动脉取血,放免法检测血浆中肾素、血管紧张素Ⅱ、醛固酮含量。结果:与模型组比较,药物干预1、2周益气组、温阳+益气组、卡托普利组大鼠血液肾素、血管紧张素Ⅱ、醛固酮含量均降低(P<0.05),药物干预4周温阳组、益气组、温阳+益气组、卡托普利组大鼠血液肾素、血管紧张素Ⅱ、醛固酮含量均降低(P<0.05)。结论:温阳药物抑制血液肾素、血管紧张素Ⅱ、醛固酮过度激活的作用较益气药物弱,随着用药时间的延长,益气及温阳药物抑制肾素、血管紧张素Ⅱ、醛固酮过度激活的作用逐渐增强。

Objective： To compare the effects of warming yang prescription with invigorating qi prescription on renin, angiotensin Ⅱ, and aldosterone at different time phase in plasma of rats with heart failure. The changes of each index were recorded with the drug-taking process. This study aimed to provide a basis for the clinical treatment of drug-taking period for the chronic heart failure. Methods： The rat model of heart failure after myocardial infarction was established by coronary artery ligation. Based on the left ventricle ejection fraction, three batches of rats were randomly divided into model group, warming yang group（the dry extractum of Radix Aconiti Lateralis Preparata and Cortex Cinnamomi）, invigorating qi group（the dry extractum of Radix Ginseng and Radix Astragali seu Hedysari）, compatibility of warming yang and invigorating qi group（mixing the dry extractum of Radix Aconiti Lateralis Preparata and Cortex Cinnamomi∶ Radix Ginseng and Radix Astragali seu Hedysari as 1∶1 ratio）, captopril group, digoxin group, and sham operation group, 6 rats in each group. After modeling, the corresponding drugs were respectively given by gavage once a day. The blood from three batches of rats was respectively collected with abdominal aortic method after one, two and four weeks. The levels of renin, angiotensin Ⅱ and aldosterone in the plasma were detected by radioimmunoassay. Results： After treatment for one and two weeks, the levels of renin, angiotensin Ⅱ and aldosterone in the invigorating qi group, the compatibility of warming yang and invigorating qi group and the captopril group were all lower than those in the model group（P〈0.05）. After treatment for four weeks, the levels of renin, angiotensin Ⅱ and aldosterone in the warming yang group, the invigorating qi group, the compatibility of warming yang and invigorating qi group and the captopril group were all lower than those in the model group（P〈0.05）. Conclusion： The inhibiting effect of warming yang prescription is weaker than that of invigorating qi prescription on excessive activation of renin, angiotensin Ⅱ and aldosterone. Nonetheless, the inhibiting effects of both kinds of prescriptions are enhanced with the extension of intervention time.