藤莪清瘀方提取物对人胃癌BGC-823细胞抑制和凋亡影响的研究

Experimental study of inhibitory and apoptosis effects of extract of Teng'e Qingyu Formula on human gastric cancer cell line BGC-823

目的:探讨藤莪清瘀方对人胃癌BGC-823细胞株的抑制及凋亡诱导作用。方法:采用不同浓度的藤莪清瘀方提取物(25.00、12.50、6.25、3.12、1.25mg/m L)体外处理人胃癌BGC-823细胞24、48、72h;采用四甲基偶氮唑蓝(MTT)法观察藤梨根不同浓度和不同作用时间对人胃癌BGC-823细胞增殖的抑制率;采用Annexin VFITC/PI双染法检测细胞凋亡情况。结果:藤莪清瘀方提取物原液药物浓度(50mg/m L)经稀释8倍(6.25mg/m L)后,抑制率最强(68.67±3.50)%,随着药物浓度的降低,抑制率随之下降;藤莪清瘀方对胃癌细胞作用48h抑制作用最强。藤莪清瘀方提取物处理细胞48h后,凋亡比率达33.15%。结论:藤莪清瘀方对胃癌细胞有明显抑制作用,并诱导其凋亡。

Objective： To explore the inhibitory and apoptosis inducing effects of extract of Teng＇e Qingyu Formula on gastric cancer BGC-823 cells. Methods： Gastric cancer BGC-823 cells were incubated in various concentrations of extract of Teng＇e Qingyu Formula（25.00, 12.50, 6.25, 3.12, 1.25mg/m L） in vitro for 24 h, 48 h and 72 h. Inhibition rates of different concentration of gastric cancer of Teng＇e Qingyu Formula on proliferation of BGC-823 cells were detected by using methyl thiazolyl tetrazolium（MTT） assay. Cell apoptosis was detected by using Annexin V-FITC/PI method. Results： Tumor inhibition rate of 6.25mg/m L extract of Teng＇e Qingyu Formula was the strongest（68.67±3.50）%. With the decreasing of concentration of Teng＇e Qingyu Formula, the tumor inhibition rate was also declined. After culturing cells with Teng＇e Qingyu Formula for 48 h, the inhibitory effect was the strongest and the ratio of apoptosis was 33.15%. Conclusion： Teng＇e Qingyu Formula has obviously inhibitory effect and apoptosis induction effect on gastric tumor cells.