两种消毒方法对感染性疾病研究室消毒灭菌效果评价

The evaluation of disinfection and sterilization effect for infectious disease lab using two disinfection methods

目的:探讨两种方消毒方法对石河子大学医学院感染性疾病研究室消毒灭菌效果。方法:实验室分别用常规紫外线照射、酒精喷洒法和甲醛熏蒸法消毒灭菌。比较两种消毒方法对实验室空气、物体表面细菌的消毒灭菌效果。从罗氏培养基上挑取可疑菌落做抗酸染色,使用PCR检测16Sr RNA、MTP40基因。结果:消毒后两组空气灭菌率为82.01%和97.38%;物体表面杀菌率为88.72%和99.12%,甲醛熏蒸法消毒后空气和物体表面灭菌率均高于常规法,差异有统计学意义(<0.05)。常规法消毒后,个别部位检测到人型结核分枝杆菌,甲醛熏蒸法消毒后结核分枝杆菌检测为阴性。结论:甲醛熏蒸消毒显著提高对空气和物体表面普通细菌的灭菌率,对结核分枝杆菌消灭彻底,消毒灭菌效果优于常规法。

Objective：To compare the effect of disinfection and sterilization for the infection disease laboratory of Medical College, Shihezi University using two disinfection methods.Methods：Laboratory was disinfected by conventional Ultraviolet irradiation with alcohol spraying and formaldehyde fumigation. Comparing the sterilization effect of the two disinfection methods in air and on objects＇ surface. Picked the suspected colonies from LI medium did acid-fast staining,and detected the 16SrRNA,MTP40 gene using PCR.Results：After being disinfected, sterilization rates of conventional disinfection and formaldehyde fumigation for air disinfection were average 82.01% and 97.38% respectively, the difference was statistically significant （P〈0.05）; object surface kill rates were 88.72% and 99.12% separately, the difference was ＇also statistically significant （P〈0.05）. After conventional disinfection, a few parts in the lab were detected Mycobacterlum tuberculosis, ＂after formaldehyde fumigation, detection of Mycobacterium tuberculosis was negative.Conclusion：The method of formaldehyde fumigation can significantly kill common bacteria and mycobaeterium tuberculosis in air and objects surface, the efficacy of formaldehyde fumigation is better than the conventional method of disinfection and sterilization.