电针对RA模型大鼠VEFG影响的实验研究

Effect of Electro-acupuncture on VEGF of Rheumatoid Arthritis Rats

目的：本实验以Ⅱ型胶原诱导的类风湿关节炎大鼠（CIA）为模型,通过观察电针对RA模型大鼠VEGF表达的影响,探讨电针治疗类风湿性关节炎的作用机理。方法：60只雄性的Wistar大鼠,随机取8只为正常组,其余大鼠采用Ⅱ型胶原和弗氏不完全佐剂复制类风湿性关节炎模型。造模后,从造模成功的大鼠中取24只大鼠随机分为模型组、泼尼松组和电针组,每组8只。造模后第21天开始治疗,正常组和模型组用生理盐水（0.1m L/10g）灌胃,泼尼松组用泼尼松药液（0.1m L/10g）灌胃,电针组用0.30cm×25mm的针灸针针刺大鼠双侧的＂足三里＂和＂昆仑＂穴,强度为1~2m A,频率2/100Hz,每次30min,每日1次,连续治疗10次。分别在造模前、造模第7天、造模第14天、造模第21天和治疗结束后称取大鼠的体质量,并采用ELISA法和蛋白印迹法（WB）检测血清和膝关节组织内VEGF的含量。结果：造模后的大鼠可见食量减少,体质量下降,足爪红肿,双侧踝关节肿胀,活动受限;与正常组相比,模型组大鼠的体质量增长变缓（P〈0.05）,电针组和泼尼松组与模型组相比差异不明显（P〉0.05）;与正常组相比,模型组血清和膝关节内的VEGF含量均升高（P〈0.05）;与模型组相比,经电针和泼尼松治疗后的大鼠血清和膝关节内的VEGF含量均将低（P〈0.05）;电针组与泼尼松组相比,差异不明显（P〉0.05）。结论：（1）电针可以显著改善CIA大鼠的饮食状况,增加体质量,减轻关节肿胀;（2）电针可以减少VEGF的分泌可能就是其中的机制之一。

Objective ：Autoimmune collagen type Ⅱ-induced arthritis （CIA） rat models were used to observe the influ- ence of electro- acupuncture （EA） on VEGF so as to study its underlying mechanism in improving RA. Methods ：There were 60 male Wistar rats and randomly choose 8 as normal control group, and the rest were used by infreund＇ s adjuvant and type Ⅱ collagen to induce arthritis. 24 rats which were successfully induced were randomly divided into three groups： model group, prednisolone group and electro- acupuncture（EA） group, with 8 in eaeh group. Treatment from the 21st day ,the normal group and model group were given saline solution （0. lmL/10g） to fill the stomach, and pred- nisone group was given prednisone solution （0. 1mL/10g） to fill the stomach. The EA（2/100Hz, 1 -2mA）group was applied to ＂Zusanli＂（ST36） and ＂Kunlun＂ （BL60） for 30rain, once daily for 10 times. Measure the rats body weight before and after the establishment, and at the end of the treatment. Then determine the plasma VEGF contents in serum and the knee joint cavity by ELISA and Western blot. Results：After modeling, paw swelling, ankle swelling, food volume and weight had different degrees of reduction;in comparison with rats in the control group, the rats in model group gained weight slowly （P 〈 0.05） ; compared with model group, the prednisolone group and electro - acupuncture group had no significant difference （ P 〉 0.05 ） ; in comparison with the normal group, serum VEGF levels within the knee were increased （P 〈 0.05 ） ; in comparison with the model group, the serum VEGF levels within the knee joint increased markedly after treatment （P 〈 0.05 ）, while the prednisolone group and electro - acupuncture group had no obvious difference（ P 〉 0.05 ）. Conclusion ： （ 1 ） EA can improve the CIA rats diet and joint swelling degree significantly, then has an obviously therapeutic effect on CIA rats; （2） The decreased expression of VEGF may be one of the mechanisms.