葫芦素E对人外周血单个核细胞体外活化及细胞因子表达的影响

The effects of cucurbitacin E on the in vitro activation and cytokine expression of human peripheral blood mononuclear cells

目的:分析葫芦素E(Cuc E)对二丁酸佛波醇酯(PDB)和离子霉素(Ion)刺激的人外周血单个核细胞(PBMC)活化及促炎因子表达的影响,探讨其潜在的抗炎效应及作用机制.方法:利用流式细胞术分析人外周血PBMC活化抗原CD69和CD25表达,用流式微球陈列检测白介素2(IL-2)、肿瘤坏死因子α(TNF-α)和干扰素γ(IFN-γ)表达的变化.结果:Cuc E能明显抑制PDB和Ion刺激引起的CD3+T细胞早期活化标志分子CD69和中期活化分子CD25的表达;同时,Cuc E以剂量依赖方式抑制PDB和Ion诱导的IL-2、TNF-α和IFN-γ的表达.结论:Cuc E对人外周血CD3+T细胞的活化具有明显抑制作用,同时减少炎症相关细胞因子的表达,提示其通过对适应性免疫的调控发挥抗炎效应.

Aim：This study aimed to explore the potential anti-inflammatory effect and the underlying action mechanism of cucurbitacin E （CucE）by using phorbol 12,13-dibutyrate （PDB） ＋Ionomycin （Ion）-activated human peripheral blood mononuclear cells as a model.Methods：The expression levels of activated antigens CD69 and CD25 were evaluated by fluorescent staining together with flow cytometry. The pro-inflammatory cytokines interleukin-2 （IL-2）,tumor necrosis factor-α（TNF-α）and interferon-γ（IFN-γ）were evaluated by cytometric bead array.Results：The results showed that CucE significantly＆nbsp;suppressed the expression of CD69 and CD25 on the surfaces of CD3 ＋ T cells.Meanwhile,PDB ＋Ion-induced expression of IL-2,TNF-αand IFN-γwere also dose-dependently inhibited by CucE.Conclu-sion：These results demonstrated that CucE could inhibit the activation of PDB ＋Ion-stimulated CD3 ＋ T lymphocytes in human PBMCs and decrease the pro-inflammatory cytokine expression,suggesting that it might exhibit anti-inflammatory activity via modulating the adaptive immunity.