摘要
采用HPLC对细辛挥发油提取物中马兜铃酸A进行检测分析,并对其含量测定方法进行研究.方法：以十八烷基硅烷键合硅胶为色谱分离填充剂,确定细辛挥发油提取物中马兜铃酸A的检测方法,流动相为乙腈-甲酸（1.0%,35∶65）;流速为1.0mL/min;检测波长250nm;柱温35℃.结果：该测定方法系统适用性良好,马兜铃酸A在体积浓度为0.505-2.525μg/mL范围内线性关系良好（r=0.9994）,且回收率较高,经分析比较,该细辛挥发油提取物中检测未含有马兜铃酸A.结论：该测定方法灵敏准确,适用于细辛挥发油提取物中马兜铃酸A的测定分析,细辛挥发油提取物的使用较为安全.
Establishing a HPLC method for analyzing the Aristolochie Acid A of the volatile oil from Asarum heterotropoides,this method is used to determine the content of Aristolochie Acid A in Asarum volatile oil through study on extracted Asarum volatile oil.An Agilent Hypersil ODS C18 column has been used.The mobile phase was acetonitrile and containing 0.1 % formic acid in the water（35∶65,V/V）.The flow rate was 1.0 ml/min.The UV wave length was set at 250 nm and the column temperature at30℃.Results show that applicability of the method system is good.The linear relationship between the concentration ranges at 0.505-2.525μg/ml was good.The result shows that there is not contains Aristolochie Acid A in the Asarum volatile oil.It is concluded that the method is stable and highly sensitive for determination of Aristolochie Acid A in the Asarum volatile oil,which promises the safe usage.
出处
《西南师范大学学报（自然科学版）》
CAS
北大核心
2015年第10期177-180,共4页
Journal of Southwest China Normal University(Natural Science Edition)
基金
重庆市制药过程与质量控制工程技术研究中心能力提升项目(CSTC2012gg-yyjsb10002-33)
西南大学博士基金项目(SWU110056和SWU110057)
关键词
细辛
挥发油
马兜铃酸A
HPLC
Asarum heterotropoides
volatile oil
Aristolochie Acid A
HPLC
