四逆汤对腹主动脉缩窄大鼠血压和压力反射敏感性的影响

Influence of Sini Decoction on Blood Pressure and Baroreflex Sensitivity in Rats with Abdominal Aortic Coarctation

目的研究四逆汤方对腹主动脉缩窄大鼠血压和压力反射敏感性(BRS)的影响。方法采用腹主动脉缩窄法制备高血压大鼠模型50只,造模成功后随机分为模型组(M组)、假手术组(B组)、四逆汤组(S组)、四逆汤+培哚普利组(SP组)、培哚普利组(P组)共5组,每组10只。术后4周开始灌胃,以假手术组为空白对照组,培哚普利组为阳性药物对照组,药物干预8周。用尾动脉仪连续动态测量大鼠左前肢血压。干预后8周通过股动脉插管,股静脉注射苯肾上腺素观察大鼠BRS、血压变化。结果造模后4周,与假手术组相比,各组收缩压均显著增高(P<0.001)、舒张压增高不明显(P>0.05),其中四逆汤组、四逆汤+培哚普利组舒张压增高(P<0.05)。药物干预后4周,与模型组相比,四逆汤组、四逆汤+培哚普利组、培哚普利组收缩压均明显降低(P<0.001),舒张压虽无明显降低,但与造模后血压相比有所下降(P<0.05)。药物干预后8周,与前一时间点血压相比,各组血压均无进一步降低(P>0.05)。药物干预后,与模型组相比,四逆汤组、四逆汤+培哚普利组、培哚普利组BRS明显上升(P<0.001)。结论四逆汤可改善腹主动脉缩窄大鼠的血压,可增加腹主动脉缩窄大鼠压力反射敏感性。

Objective To study the effects of Sini decoction（SND）on blood pressure and baroreflex sensitivity（BRS）in rats with abdominal aortic coarctation.Methods Fifty hypertension rats were established by abdominal aortic coarctation operation.After modeling,rats were randomly divided into 5 groups：Sham operation group,SND group,SND plus perindopril group,perindopril group and model group.Rats were gavaged 4 weeks later.The sham operation group was the control group,perindopril as the positive control group.Caudal artery with continuous dynamic measurement instrument left forelimb blood pressure in rats.By cannulating into femoral artery,injecting phenylephrine into femoral artery,we observe the change of BRS,MAP,and HR.Results After modeling of 4 weeks,compared with the control group,the systolic blood pressure were significantly increased in each group（P 0.001）.After drug intervention of 4 weeks,compared with the model group,the systolic blood pressure were significantly lower in SND group,SND plus perindopril group,perindopril group（P 0.001）.The diastolic blood pressure was lower after modeling（P〈0.05）.After drug intervention,compared with model group,the BRS obviously increased in SND group,SND plus perindopril group,perindopril group（P 0.001）.Conclusion SND can improve the blood pressure and increase the baroreflex sensitivity in rats with abdominal aortic coarctation.